在裸鼠传代鼻咽癌培养的多细胞肿瘤球体中鉴定小鼠内源性嗜异性逆转录病毒。

Identification of murine endogenous xenotropic retrovirus in cultured multicellular tumour spheroids from nude-mouse-passaged nasopharyngeal carcinoma.

作者信息

Crawford D H, Achong B G, Teich N M, Finerty S, Thompson J L, Epstein M A, Giovanella B C

出版信息

Int J Cancer. 1979 Jan 15;23(1):1-7. doi: 10.1002/ijc.2910230102.

Abstract

After nude-mouse-passage, a carcinoma of the nasopharynx was found to contain a few scattered C-type retrovirus particles. Culture of this nude-mouse-grown material over solid agar allowed the human tumour cells to grow as multicellular spheroids which increased in number by budding and could be subcultured for up to 6 months. Within the spheroids the human tumour cells expressed increased retrovirus replication and large numbers of C-type particles were observed. Treatment with a halogenated pyrimidine further enhanced the virus production. This tissue culture system provided sufficient virus production for the identification of the retrovirus as an endogenous xenotropic murine leukaemia virus and not a human agent.

摘要

经裸鼠传代后，发现一株鼻咽癌含有一些散在的C型逆转录病毒颗粒。将这种在裸鼠体内生长的材料在固体琼脂上培养，可使人类肿瘤细胞生长为多细胞球体，这些球体通过出芽增加数量，并可传代培养长达6个月。在球体内，人类肿瘤细胞的逆转录病毒复制增加，观察到大量C型颗粒。用卤代嘧啶处理进一步提高了病毒产量。该组织培养系统产生了足够的病毒用于鉴定该逆转录病毒为内源性嗜异性鼠白血病病毒而非人类病原体。