Qi Xiao-Ying, Hong Li, Guo Feng-Qin, Fu Qiong, Chen Lu, Li Bing-Shu
Department of Gynecology and Obstetrics, Renmin Hospital of Wuhan University, Jiefang Road 238, Wuhan, Hubei 430060, China.
Saudi Med J. 2011 May;32(5):474-8.
To investigate the presence of transforming growth factor-beta 1 (TGF-ß1) and connective tissue growth factor (CTGF) in women with pelvic organ prolapse (POP).
This study was conducted from May to December 2009. Fifty patients with POP that underwent vaginal hysterectomy in the Department of Gynecology, Renmin Hospital of Wuhan University, Hubei, Wuhan, China were enrolled in this study. They were divided into: Group 1 (n=10); Group 2 (n=10); and Group 3 (n=10) according to Pelvic Organ Prolapse Quantitation (POP-Q). Meanwhile, 20 cases treated by vaginal hysterectomy for other benign gynecological diseases were selected as the control group. Immunohistochemical staining and Western blot were performed to detect the expression of TGF-ß1 and CTGF.
Immunohistochemical staining of TGF-ß1 and CTGF were mainly expressed in the cytoplasm of fibroblast cells. The expression of TGF-ß1 and CTGF protein was significantly negatively correlated with POP-Q stage. There were significantly positive correlations between the expression of TGF-ß1 and CTGF protein. The expression of TGF-ß1 protein among the 3 POP groups were all significantly lower than that of the control group, while there was no significant differences in the expression of TGF-ß1 protein among the POP groups, excluding the comparison between Groups 1 and 3. The expression of CTGF protein in the 3 POP groups were all significantly lower than that of the control group, and significant differences were also detected among the 3 POP groups.
In this study, we found that the TGF-ß1 and CTGF protein expression may be associated with POP, especially in POP-Q stages.
探讨盆腔器官脱垂（POP）女性体内转化生长因子-β1（TGF-β1）和结缔组织生长因子（CTGF）的存在情况。
本研究于2009年5月至12月进行。选取在武汉大学人民医院妇科接受阴道子宫切除术的50例POP患者纳入本研究。根据盆腔器官脱垂定量分期（POP-Q）将她们分为：第1组（n = 10）；第2组（n = 10）；第3组（n = 10）。同时，选取20例因其他良性妇科疾病接受阴道子宫切除术的患者作为对照组。采用免疫组织化学染色和蛋白质印迹法检测TGF-β1和CTGF的表达。
TGF-β1和CTGF的免疫组织化学染色主要在成纤维细胞的细胞质中表达。TGF-β1和CTGF蛋白的表达与POP-Q分期呈显著负相关。TGF-β1和CTGF蛋白的表达之间存在显著正相关。3个POP组中TGF-β1蛋白的表达均显著低于对照组，而POP组之间TGF-β1蛋白的表达无显著差异，但第1组和第3组之间的比较除外。3个POP组中CTGF蛋白的表达均显著低于对照组，且3个POP组之间也检测到显著差异。
在本研究中，我们发现TGF-β1和CTGF蛋白表达可能与POP相关，尤其是在POP-Q分期中。
