Spalletticernia D, Dagnano I, Salvatore C, Portella G, Zupi G, Vecchio G, Laccetti P
UNIV NAPLES,FAC MED & CHIRURG,DIPARTIMENTO BIOL & PATOL CELLULARE & MOLEC,CNR,I-80131 NAPLES,ITALY. CNR,IST TECNOL BIOMED,I-00161 ROME,ITALY. REGINA ELENA INST CANC RES,CTR EXPTL RES,CHEMIOTERAPIA SPERIMENTALE PRECLIN LAB,I-00158 ROME,ITALY.
Int J Oncol. 1995 Mar;6(3):647-54. doi: 10.3892/ijo.6.3.647.
The effects exerted by ms transformation upon sensitivity to anticancer drugs by using an epithelial cell system displaying different levels of ras expression which well correlate with different degrees of cell malignancy have been evaluated. The diminished drug accumulation of the transformed cell lines was found to be correlated with a depolarization of the cell membrane as observed by a flow cytometric analysis and not correlated with the multidrug resistance (mdr) protein. The most resistant cell line, MPTK-6, showed the lowest drug accumulation levels and the lowest membrane potential. These observations indicate that ras neoplastic transformation of the rat thyroid epithelial cells modifies the membrane potential and this modification is possibly involved in anticancer drugs resistance.
利用显示不同水平ras表达的上皮细胞系统（其与不同程度的细胞恶性程度密切相关），评估了ras转化对癌细胞对抗癌药物敏感性的影响。通过流式细胞术分析观察到，转化细胞系药物蓄积减少与细胞膜去极化相关，而与多药耐药（mdr）蛋白无关。耐药性最强的细胞系MPTK - 6显示出最低的药物蓄积水平和最低的膜电位。这些观察结果表明，大鼠甲状腺上皮细胞的ras肿瘤转化改变了膜电位，这种改变可能与抗癌药物耐药性有关。
