Naujocks G, Schmitz A, Schramm J, Wiestler O, Schirrmacher V
GERMAN CANC RES CTR,DIV CELLULAR IMMUNOL,D-69120 HEIDELBERG,GERMANY. UNIV BONN,MED CTR,DEPT NEUROSURG,W-5300 BONN,GERMANY. UNIV BONN,MED CTR,DEPT NEUROPATHOL,W-5300 BONN,GERMANY.
Int J Oncol. 1995 Apr;6(4):759-65. doi: 10.3892/ijo.6.4.759.
Using two malignant rat glioma cell lines, we tested to what extent peripheral immunization could affect tumor growth in the brain of syngeneic rats. Peripheral subcutaneous (s.c.) immunization was performed with autologous Newcastle-disease-virus (NDV)-infected or non-infected live tumor cells. Thus immunized rats or non-immunized controls were intracerebrally implanted with increasing numbers of the respective malignant glioma cells. Without immunization the mean survival time after intracerebral implantation of 1x10(4) TZ363 or RG2 glioma cells was 9 and 29 days respectively. After s.c. immunization with either NDV-infected or non-infected TZ363 cells only 25% or less of challenged animals developed tumors in the brain. Immunization with NDV only had no effect. In RG2 glioma, s.c. immunization had no effect on tumor growth in the central nervous system and on survival time, no matter what kind of vaccine was used. These results clearly show, that in principle the efferent arm of the anti-tumor response can be effective accross the blood-brain barrier and extend into the microenvironment of the central nervous system. Whether or not glioma lines can induce this immunity and respond to it, seems to depend on their individual immunobiological characteristics.
我们使用两种恶性大鼠胶质瘤细胞系，测试了外周免疫在多大程度上会影响同基因大鼠脑内肿瘤的生长。采用自体新城疫病毒（NDV）感染或未感染的活肿瘤细胞进行外周皮下（s.c.）免疫。对如此免疫的大鼠或未免疫的对照大鼠脑内植入数量递增的相应恶性胶质瘤细胞。未免疫时，脑内植入1×10⁴个TZ363或RG2胶质瘤细胞后的平均存活时间分别为9天和29天。用NDV感染或未感染的TZ363细胞进行皮下免疫后，只有25%或更少的受攻击动物脑内出现肿瘤。仅用NDV免疫没有效果。在RG2胶质瘤中，皮下免疫对中枢神经系统的肿瘤生长和存活时间均无影响，无论使用何种疫苗。这些结果清楚地表明，原则上抗肿瘤反应的传出臂可以有效穿过血脑屏障并延伸至中枢神经系统的微环境。胶质瘤细胞系是否能够诱导这种免疫并对其作出反应，似乎取决于它们各自的免疫生物学特性。
