Department of Oral Pathology and Oral Medicine, The Maurice and Gabriela Goldschleger School of Dental Medicine, Tel Aviv University, Israel.
Cancer Cytopathol. 2011 Aug 25;119(4):235-46. doi: 10.1002/cncy.20157. Epub 2011 May 6.
Most cases of laryngeal cancer are preceded by precursor lesions which, if left untreated, can progress toward an invasive cancer. The objective of this study was to investigate the presence of chromosomal numerical aberrations in cells that were collected by noninvasive brush sampling from laryngeal lesions.
Laryngeal brush samples from 52 patients were analyzed simultaneously for morphology and fluorescence in situ hybridization (FISH) using centromeric probes for chromosome 17, chromosome 8, and a locus-specific instability (LSI) v-myc avian myelocytomatosis viral oncogene homolog (myc) proto-oncogene protein (C-MYC) probe for the MYC gene. The patients were divided according to histopathologic diagnosis. Group 1 included patients with squamous cell carcinoma, carcinoma in situ, and severe dysplasia; Group 2 included patients with moderate dysplasia, mild dysplasia, and hyperplasia; and Group 3 included patients with benign nondysplastic lesions.
The proportion of cells with MYC and chromosome 8 gains demonstrated significant trends toward being the highest in Group 1 and the lowest in Group 3 (P = .001 and P = .003, respectively). No significant trend was observed for chromosome 17. Mann-Whitney Bonferroni-corrected analyses revealed that the most significant contribution was the difference between Groups 1 and 3 (P = .0195 for MYC gains and P = .036 for chromosome 8 gains). When using a cutoff point of 4% aneuploid cells (ACs), both MYC and chromosome 8 differed significantly between groups (P = .030 and P = .037, respectively).
The current results suggested that FISH analysis of brush samples obtained noninvasively from suspicious laryngeal lesions can augment the clinical examination in predicting the nature of the lesions and can aid clinicians in monitoring and follow-up of high-risk patients. Cancer (Cancer Cytopathol) 2011. © 2011 American Cancer Society.
大多数喉癌病例之前都有前驱病变，如果不加以治疗，这些病变可能会进展为浸润性癌。本研究的目的是探讨通过非侵入性刷取取样从喉病变中收集的细胞中是否存在染色体数量异常。
同时对 52 例患者的喉刷样本进行形态学和荧光原位杂交（FISH）分析，使用染色体 17、8 号染色体和特定染色体不稳定（LSI）v-myc 禽髓细胞瘤病毒致癌基因同源物（myc）原癌基因蛋白（C-MYC）探针检测 MYC 基因的中心粒探针。根据组织病理学诊断将患者分为三组。第 1 组包括鳞状细胞癌、原位癌和重度不典型增生患者；第 2 组包括中度不典型增生、轻度不典型增生和增生患者；第 3 组包括良性非异型病变患者。
MYC 和 8 号染色体获得的细胞比例显示出在第 1 组中最高，在第 3 组中最低的显著趋势（P =.001 和 P =.003）。染色体 17 没有观察到显著趋势。曼-惠特尼 Bonferroni 校正分析显示，最显著的差异是第 1 组和第 3 组之间的差异（MYC 获得的 P =.0195，8 号染色体获得的 P =.036）。当使用 4%的非整倍体细胞（AC）截断点时，MYC 和 8 号染色体在组间差异均有统计学意义（P =.030 和 P =.037）。
目前的结果表明，从可疑喉病变非侵入性刷取样本进行 FISH 分析可以增强临床检查，预测病变性质，并帮助临床医生监测和随访高危患者。癌症（癌症细胞病理学）2011。© 2011 年美国癌症协会。
