Laboratoire de Spectroscopie Vibrationnelle et Electrochimie des Biomolécules (Institut de Chimie, UdS), Strasbourg, France.
J Phys Chem B. 2011 Jun 2;115(21):7165-70. doi: 10.1021/jp202656w. Epub 2011 May 10.
The electrochemical behavior of three proteins fragments from the respiratory chain of the extremophilic bacterium Thermus thermophilus , namely, cytochrome c(1) (Cyt-c(1)), cytochrome c(552) (Cyt-c(552)), and Cu(A), immobilized on three-dimensional gold nanoparticles electrodes was investigated by cyclic voltammetry. The gold nanoparticles were modified by either dithiobissuccinimidyl propionate (DTSP) or a mixed self-assembled monolayer of 6-mercaptohexan-1-ol and hexanethiol, depending on the surface of the protein. High surface coverages with enzymes and good electron transfer rates were achieved in the case of Cyt-c(1) immobilized on DTSP-modified gold nanoparticles and Cyt-c(552) or Cu(A) immobilized on mixed SAMs-modified gold nanoparticles. Interestingly, high surface coverages with Cu(A) were also observed on DTSP-modified gold nanoparticles, but a slower electron transfer rate was determined in this case. The gold nanoparticle/protein assemblies were characterized by surface-enhanced IR spectroscopy and transmission electron microscopy.
通过循环伏安法研究了极端嗜热菌 Thermus thermophilus 呼吸链中三种蛋白质片段的电化学行为，即细胞色素 c(1)（Cyt-c(1)）、细胞色素 c(552)（Cyt-c(552)）和 Cu(A)，它们固定在三维金纳米粒子电极上。金纳米粒子通过二硫代琥珀酰亚胺基丙酸酯（DTSP）或 6-巯基己醇和己硫醇的混合自组装单层进行修饰，具体取决于蛋白质的表面。在 DTSP 修饰的金纳米粒子上固定 Cyt-c(1)以及在混合 SAMs 修饰的金纳米粒子上固定 Cyt-c(552)或 Cu(A)的情况下，实现了高的酶表面覆盖率和良好的电子转移速率。有趣的是，在 DTSP 修饰的金纳米粒子上也观察到了高的 Cu(A)表面覆盖率，但在这种情况下确定的电子转移速率较慢。金纳米粒子/蛋白质组装体通过表面增强红外光谱和透射电子显微镜进行了表征。
