Tung Fu I, Chiu Chih T, Chang Yi P, Wang Yng J
Department of Orthopedic Surgery, Yang Ming Branch, Taipei City Hospital, Taiwan, ROC.
Artif Cells Blood Substit Immobil Biotechnol. 2011 Oct;39(5):335-40. doi: 10.3109/10731199.2011.574634. Epub 2011 May 11.
Fibers comprised of reconstituted type I collagen were prepared by a gravity filament forming process and crosslinked with 0.1% glutaraldehyde. These fibers have a crosslinking index of about 90% (89.89 ± 1.82%) with higher denature temperature (74.43 ± 0.08°C) as compared to that without glutaraldehyde treatment (52.1 ± 0.17°C). The ultimate tensile strength of the collagen fibers increases from 99.4 ± 12.9 to 174.4 ± 9.0 MPa after glutaraldehyde-crosslinking. L929 fibroblast cells were seeded and cultured using these newly developed collagen fibers. The fibroblast cells proliferated well and covered all surface areas of the collagen fiber. These collagen fibers have a great potential for application in 3-D tissue engineering.
由重组I型胶原构成的纤维通过重力长丝形成工艺制备，并与0.1%的戊二醛交联。与未经戊二醛处理的纤维（52.1±0.17°C）相比，这些纤维的交联指数约为90%（89.89±1.82%），变性温度更高（74.43±0.08°C）。戊二醛交联后，胶原纤维的极限拉伸强度从99.4±12.9MPa增加到174.4±9.0MPa。使用这些新开发的胶原纤维接种并培养L929成纤维细胞。成纤维细胞增殖良好，覆盖了胶原纤维的所有表面区域。这些胶原纤维在三维组织工程中具有巨大的应用潜力。
