Graduate School of Science and Engineering, University of Toyama.
Microbes Environ. 2008;23(1):40-3. doi: 10.1264/jsme2.23.40.
The cellular slime mold Dictyostelium discoideum grows as unicellular free-living amoebae in the presence of nutrients. Upon starvation, the amoebae aggregate and form multicellular structures that each consist of a stalk and spores. D. discoideum encodes at least four proteins (Sir2A, Sir2B, Sir2C, and Sir2D) homologous to human SIRT. RT-PCR and WISH analyses showed that the genes for Sir2A, Sir2C, and Sir2D were expressed at high levels in growing cells but at decreased levels in developing cells, whereas the gene encoding Sir2B was expressed in the prestalk-cell region in the developmental phase.
细胞黏菌 D. discoideum 在有营养物质存在的情况下作为单细胞自由生活的变形虫生长。在饥饿时，变形虫聚集并形成每个由柄部和孢子组成的多细胞结构。D. discoideum 编码至少四种与人类 SIRT 同源的蛋白质（Sir2A、Sir2B、Sir2C 和 Sir2D）。RT-PCR 和 WISH 分析表明，Sir2A、Sir2C 和 Sir2D 的基因在生长细胞中高水平表达，但在发育细胞中水平降低，而编码 Sir2B 的基因在发育阶段的前柄细胞区表达。
