Will C, Wilhelm O, Hohl S, Mobus V, Weidle U, Kreienberg R, Janicke F, Schmitt M, Graeff H
TECH UNIV MUNICH, KLINIKUM RECHTS ISAR, FRAUENKLIN, D-81675 MUNICH, GERMANY. UNIV ULM, FRAUENKLIN, D-89075 ULM, GERMANY. BOEHRINGER MANNHEIM GMBH, D-82377 PENZBERG, GERMANY.
Int J Oncol. 1994 Oct;5(4):753-61. doi: 10.3892/ijo.5.4.753.
Expression of urokinase-type plasminogen activator (uPA), tissue-type plasminogen activator (t-PA), their inhibitor PAI-1 and the uPA-receptor (uPAR) was characterized in six human tumor cell lines (OV-MZ-6, -10, -13, -15, -19 and OVCAR-3) established from patients with cystadenocarcinoma of the ovary. The invasive potential of the ovarian cancer cell lines determined in an in vitro invasion assay did neither correlate with the antigen level of uPA, t-PA, PAI-1 or uPAR nor with the cell surface uPA activity, however, did correlate with the cell surface-bound plasmin activity. The in vitro invasiveness of three cancer cell lines selected displaying a different pattern of uPA and uPAR expression was significantly inhibited by a recombinant soluble truncated form of the uPAR functioning as a scavenger for uPA. Our results suggest that the interference of the uPA/uPAR interaction leads to a reduced in vitro invasiveness of human ovarian cancer cells independent of the level of uPA and uPAR expression.
在从卵巢囊腺癌患者体内建立的六种人肿瘤细胞系（OV-MZ-6、-10、-13、-15、-19和OVCAR-3）中，对尿激酶型纤溶酶原激活物（uPA）、组织型纤溶酶原激活物（t-PA）、它们的抑制剂PAI-1以及uPA受体（uPAR）的表达进行了表征。在体外侵袭试验中测定的卵巢癌细胞系的侵袭潜能，既不与uPA、t-PA、PAI-1或uPAR的抗原水平相关，也不与细胞表面uPA活性相关，然而，却与细胞表面结合的纤溶酶活性相关。选择的三种显示不同uPA和uPAR表达模式的癌细胞系的体外侵袭性，被一种作为uPA清除剂的重组可溶性截短形式的uPAR显著抑制。我们的结果表明，uPA/uPAR相互作用的干扰导致人卵巢癌细胞体外侵袭性降低，且与uPA和uPAR的表达水平无关。
