通过离心制备仓鼠和小鼠卵的有核和无核片段。
Preparation of nucleate and anucleate fragments of hamster and mouse eggs by centrifugation.
作者信息
Yanagimachi R, Yang C H
机构信息
Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822.
出版信息
J Exp Zool. 1990 Feb;253(2):220-5. doi: 10.1002/jez.1402530212.
PMID:2155991
Abstract
Nucleate and anucleate fragments of hamster and mouse eggs can be prepared by centrifuging zona-free eggs in a Percoll gradient. These fragments are fertilizable and can be used for various studies of nucleus-ooplasm interactions.
摘要
通过在Percoll梯度中对去透明带的仓鼠和小鼠卵子进行离心，可以制备出有核和无核碎片。这些碎片具有受精能力，可用于细胞核与细胞质相互作用的各种研究。
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