评估一种非平衡等电聚焦（IEF）方法用于同时分型酯酶D（EsD）、红细胞酸性磷酸酶（AcP1）、磷酸葡萄糖变位酶（PGM1）、腺苷酸激酶（AK）和腺苷脱氨酶（ADA）。

Evaluation of a nonequilibrium isoelectric focusing (IEF) method for the simultaneous typing of esterase D (EsD), red cell acid phosphatase (AcP1), phosphoglucomutase (PGM1), adenylate kinase (AK), and adenosine deaminase (ADA).

作者信息

Stockwell D C, Gregonis D J, Jones D T

机构信息

San Bernardino County Sheriff's Regional Forensic Science Laboratory, CA.

出版信息

J Forensic Sci. 1990 Jan;35(1):46-61.

PMID:2155993

Abstract

A nonequilibrium isoelectric focusing method incorporating the chemical spacers MOPS and HEPES was developed and subsequently evaluated for its ability to reliably discriminate common and rare phenotypes in the esterase D (EsD), red cell acid phosphatase (AcP1), phosphoglucomutase (PGM1), adenylate kinase (AK), and adenosine deaminase (ADA) isoenzyme systems. The validation procedures used were blind testing, comparison of results to conventional methods, and evaluation of known rare variant phenotypes. This method proved to be a quick and reliable method for typing all five isoenzyme systems, while providing an excellent probability of discrimination (PD = 0.96).

摘要

开发了一种结合化学间隔物MOPS和HEPES的非平衡等电聚焦方法，并随后评估了其在酯酶D（EsD）、红细胞酸性磷酸酶（AcP1）、磷酸葡萄糖变位酶（PGM1）、腺苷酸激酶（AK）和腺苷脱氨酶（ADA）同工酶系统中可靠区分常见和罕见表型的能力。所采用的验证程序包括盲测、将结果与传统方法进行比较以及对已知罕见变异表型进行评估。该方法被证明是对所有五个同工酶系统进行分型的快速可靠方法，同时提供了出色的鉴别概率（PD = 0.96）。