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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Butler M, Darbre A, Arnstein H R

Biochem J. 1975 Sep;150(3):419-32. doi: 10.1042/bj1500419.

tRNA was extracted from rabbit liver by both the phenol and diethyl pyrocarbonate methods under conditions preventing deacylation of the amino acids attached in vivo. 2. After deacylation 12 amino acids were determined by gas-liquid chromatography, by using the flame-ionization and nitrogen-sensitive thermionic detectors. 3. Comparison of the distribution of 12 amino acids attached to tRNA with those contained in total tissue protein and in the free pool showed little correlation. 4. Results for the enzymic charging assay for tRNA in vitro did not correlate satisfactorily with the analysis of amino acids attached to tRNA in vivo. Marked differences were ntoed in comparison made between our own and other published results.

在防止体内连接的氨基酸脱酰基的条件下，采用苯酚法和焦碳酸二乙酯法从兔肝脏中提取tRNA。2. 脱酰基后，使用火焰离子化检测器和氮敏感热离子检测器通过气液色谱法测定12种氨基酸。3. 比较连接在tRNA上的12种氨基酸的分布与总组织蛋白和游离库中所含氨基酸的分布，发现相关性很小。4. tRNA体外酶促充电试验的结果与体内连接在tRNA上的氨基酸分析结果相关性不令人满意。我们自己的结果与其他已发表的结果相比存在明显差异。

Butler M, Darbre A, Arnstein H R

Biochem J. 1975 Sep;150(3):419-32. doi: 10.1042/bj1500419.

tRNA was extracted from rabbit liver by both the phenol and diethyl pyrocarbonate methods under conditions preventing deacylation of the amino acids attached in vivo. 2. After deacylation 12 amino acids were determined by gas-liquid chromatography, by using the flame-ionization and nitrogen-sensitive thermionic detectors. 3. Comparison of the distribution of 12 amino acids attached to tRNA with those contained in total tissue protein and in the free pool showed little correlation. 4. Results for the enzymic charging assay for tRNA in vitro did not correlate satisfactorily with the analysis of amino acids attached to tRNA in vivo. Marked differences were ntoed in comparison made between our own and other published results.

在防止体内连接的氨基酸脱酰基的条件下，采用苯酚法和焦碳酸二乙酯法从兔肝脏中提取tRNA。2. 脱酰基后，使用火焰离子化检测器和氮敏感热离子检测器通过气液色谱法测定12种氨基酸。3. 比较连接在tRNA上的12种氨基酸的分布与总组织蛋白和游离库中所含氨基酸的分布，发现相关性很小。4. tRNA体外酶促充电试验的结果与体内连接在tRNA上的氨基酸分析结果相关性不令人满意。我们自己的结果与其他已发表的结果相比存在明显差异。