Li Xia, Jiang Zheng-Lin, Wang Guo-Hua
Institute of Nautical Medicine, Nantong University, Nantong 226001, China.
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2011 Feb;27(1):46-50.
To measure the content of arginine vasopressin (AVP) and V1b receptor expression in the brain areas in rats of both genders and after rotatory stimulation and thereby, to identify the involvement of AVP in the mechanisms of motion sickness.
SD rats were rotated about a horizontal axis for 30 min, the content of AVP and the expression of V1b receptors in some brain areas were then measured with radioimmunological analysis and immunofluorescent method respectively.
We proved that: (1) In female rats, the content of AVP in each area we measured in rotation group did not show any significant change compared with that in control group (P > 0.05). In male rats, the AVP content of control group in each area was higher than that of female rats, but reduced by rotatory stimulation in forebrain, diencephalon and pontine (P < 0.05 or 0.01), however, the changes in the cerebellum and medulla of rotation group were not significant (P > 0.05). (2) The positive cell number of V1b receptor expression in the supraoptic nucleus of female rats in rotation group was lower, but higher in the vestibular nucleus and area postrema than that in control group (P < 0.05 or 0.01). In male rats, the V1b receptor positive cell number in the supraoptic nucleus and vestibular nucleus of rotation group did not show significant change compared with that of control group (P > 0.05), but a slight increase in the medulla of rotation group rats was observed (P < 0.05).
The gender difference in the susceptibility of motion sickness is potentially associated with the discrepancies in AVP content in the forebrain, diencephalon and pontine, in the expression of AVP-V1 receptors in the vestibular nucleus and area postrema, and in responses to rotatory stimulation, and that the vestibular nucleus and area postrema may be the areas targeted by AVP V1 receptor antagonist for antimotion sickness.
测量旋转刺激后雌雄大鼠脑区中精氨酸加压素（AVP）的含量及V1b受体的表达，从而确定AVP在晕动病机制中的作用。
将SD大鼠绕水平轴旋转30分钟，然后分别用放射免疫分析法和免疫荧光法测量部分脑区中AVP的含量及V1b受体的表达。
我们证实：（1）在雌性大鼠中，旋转组各测量脑区的AVP含量与对照组相比无显著变化（P>0.05）。在雄性大鼠中，各脑区对照组的AVP含量高于雌性大鼠，但前脑、间脑和脑桥经旋转刺激后AVP含量降低（P<0.05或0.01），然而，旋转组小脑和延髓的变化不显著（P>0.05）。（2）旋转组雌性大鼠视上核中V1b受体表达的阳性细胞数低于对照组，但前庭核和最后区高于对照组（P<0.05或0.01）。在雄性大鼠中，旋转组视上核和前庭核中V1b受体阳性细胞数与对照组相比无显著变化（P>0.05），但旋转组大鼠延髓中V1b受体阳性细胞数略有增加（P<0.05）。
晕动病易感性的性别差异可能与前脑、间脑和脑桥中AVP含量、前庭核和最后区中AVP-V1受体表达以及对旋转刺激反应的差异有关，且前庭核和最后区可能是AVP V1受体拮抗剂抗晕动病的作用靶点。
Zotero 插件