Scherzer P, Wald H, Popovtzer M M
Nephrology Service, Hadassah University Hospital, Jerusalem, Israel.
Kidney Int. 1990 Mar;37(3):870-4. doi: 10.1038/ki.1990.60.
To further characterize changes in tubular Na-K-ATPase in acute tubular necrosis (ATN), segmental analysis was performed in rat nephrons. Na-K-ATPase was assayed in the following segments: proximal convolution (PC), proximal straight (PS), outer medullary thick ascending limb (MTAL), cortical thick ascending limb (CTAL), distal convolution (DC) and cortical collecting duct (CCD) in three groups of rats: 1.) intact; 2.) moderate non-oliguric ATN; and 3.) severe oliguric ATN. GFR and CNa/GFR X 100 were in group 1 0.80 +/- 0.05 ml/min and 0.68 +/- 0.06, in group 2 0.14 +/- 0.02 and 1.46 +/- 0.35, and in group 3 0.04 +/- 0.01 and 0.46 +/- 0.15, respectively. Na-K-ATPase in PC and PS were similar in all three groups. Na-K-ATPase levels were in MTAL: in group 1 37 +/- 2 X 10(-11) mol/mm/min, in group 2 20 +/- 1 X 10(-11), P less than 0.001 versus group 1, and in group 3 24 +/- 2 X 10(-11), P less than 0.001 versus group 1. In CTAL Na-K-ATPase levels were: in group 1 40 +/- 2 X 10(-11), in group 2 33 +/- 1 X 10(-11), P less than 0.001 versus group 1, and in group 3 27 +/- 2 X 10(-11), P less than 0.001 versus groups 1 and 2.(ABSTRACT TRUNCATED AT 250 WORDS)
为了进一步描述急性肾小管坏死（ATN）时肾小管钠钾ATP酶的变化特征，对大鼠肾单位进行了节段分析。在三组大鼠的以下节段检测钠钾ATP酶：近曲小管（PC）、近直小管（PS）、外髓质厚升支（MTAL）、皮质厚升支（CTAL）、远曲小管（DC）和皮质集合管（CCD）：1.）完整组；2.）中度非少尿型ATN组；3.）重度少尿型ATN组。第一组的肾小球滤过率（GFR）和CNa/GFR×100分别为0.80±0.05ml/min和0.68±0.06，第二组分别为0.14±0.02和1.46±0.35，第三组分别为0.04±0.01和0.46±0.15。三组大鼠PC和PS中的钠钾ATP酶相似。MTAL中的钠钾ATP酶水平：第一组为37±2×10⁻¹¹mol/mm/min，第二组为20±1×10⁻¹¹，与第一组相比P<0.001，第三组为24±2×10⁻¹¹，与第一组相比P<0.001。CTAL中的钠钾ATP酶水平：第一组为40±2×10⁻¹¹，第二组为33±1×10⁻¹¹，与第一组相比P<0.001，第三组为27±2×10⁻¹¹，与第一组和第二组相比P<0.001。（摘要截选至250字）
