Souchie Edson L, Azcón Rosario, Barea Jose M, Silva Eliane M R, Saggin-Júnior Orivaldo J
Instituto Federal de Educação, Ciência e Tecnologia Goiano, Rio Verde, GO, Brazil.
An Acad Bras Cienc. 2010 Sep;82(3):771-7. doi: 10.1590/s0001-37652010000300023.
This study evaluated the synergism between several P-solubilizing fungi isolates and arbuscular mycorrhizal fungi to improve clover ( Trifolium pratense) growth in the presence of Araxá apatite. Clover was sown directly in plastic pots with 300g of sterilized washed sand, vermiculite and sepiolite 1:1:1 (v:v:v) as substrate, and grown in a controlled environment chamber. The substrate was fertilized with 3 g L(-1) of Araxá apatite. A completely randomized design, in 8×2 factorial scheme (eight P-solubilizing fungi treatments with or without arbuscular mycorrhizal fungi)and four replicates were used. The P-solubilizing fungi treatments consisted of five Brazilian P-solubilizing fungi isolates (PSF 7, 9, 20, 21 and 22), two Spanish isolates ( Aspergillus niger and the yeast Yarowia lipolytica) and control (non-inoculated treatment). The greatest clover growth rate was recorded when Aspergillus niger and PSF 21 were co-inoculated with arbuscular mycorrhizal fungi. Aspergillus niger, PSF 7 and PSF 21 were the most effective isolates on increasing clover growth in the presence of arbuscular mycorrhizal fungi. Greater mycorrhizal colonization resulted in greater clover growth rate in most PSF treatments. PSF 7 was the best isolate to improve the establishment of mycorrhizal and rhizobia symbiosis.
本研究评估了几种解磷真菌分离株与丛枝菌根真菌之间的协同作用，以改善在阿拉沙磷灰石存在下红三叶草（Trifolium pratense）的生长。将红三叶草直接播种在装有300克经过消毒洗涤的沙子、蛭石和海泡石（1:1:1，体积比）作为基质的塑料盆中，并在可控环境箱中生长。向基质中施加3克/升的阿拉沙磷灰石。采用完全随机设计，8×2析因方案（八种解磷真菌处理，有无丛枝菌根真菌），重复四次。解磷真菌处理包括五种巴西解磷真菌分离株（PSF 7、9、20、21和22）、两种西班牙分离株（黑曲霉和酵母解脂耶氏酵母）以及对照（未接种处理）。当黑曲霉和PSF 21与丛枝菌根真菌共同接种时，红三叶草的生长速率最高。在丛枝菌根真菌存在的情况下，黑曲霉、PSF 7和PSF 21是促进红三叶草生长最有效的分离株。在大多数PSF处理中，更高的菌根定殖率导致红三叶草生长速率更高。PSF 7是改善菌根与根瘤菌共生建立的最佳分离株。
