USDA-ARS, 1636 E Alisal Street, Salinas, CA 93905, USA.
Arch Virol. 2011 Sep;156(9):1635-40. doi: 10.1007/s00705-011-1009-9. Epub 2011 May 12.
A virus associated with yellow-to-purple ringspot on honeysuckle plants has been detected and tentatively named honeysuckle ringspot virus (HnRSV). The complete nucleotide sequence of HnRSV from infected honeysuckle has been determined. The genomic RNA of HnRSV is 3,956 nucleotides in length and is predicted to contain five open reading frames (ORFs). Comparisons of the amino acid sequences of the ORFs of HnRSV with those of members of the family Tombusviridae show that HnRSV is closely related to members of the genus Carmovirus. Phylogenetic analysis based on the amino acid sequences of RdRp and coat protein and nucleotide sequences of the whole genome revealed that HnRSV forms a subgroup with the carmoviruses. Together, our results support the classification of HnRSV as a member of a new species in the genus Carmovirus, family Tombusviridae.
已检测到与忍冬植物黄紫环斑相关的病毒，并暂定名为忍冬环斑病毒（HnRSV）。已确定来自受感染忍冬的 HnRSV 的完整核苷酸序列。HnRSV 的基因组 RNA 长 3956 个核苷酸，预计包含五个开放阅读框（ORF）。HnRSV 的 ORF 氨基酸序列与 Tymoviridae 家族成员的氨基酸序列比较表明，HnRSV 与 Carmovirus 属的成员密切相关。基于 RdRp 和外壳蛋白的氨基酸序列以及整个基因组的核苷酸序列进行的系统发育分析表明，HnRSV 与 carmoviruses 形成一个亚群。综上所述，我们的研究结果支持将 HnRSV 分类为 Tymoviridae 家族 Carmovirus 属的一个新种成员。
