Department of Botany, Oklahoma State University, Stillwater, OK 74078, USA.
Virus Res. 2011 Sep;160(1-2):256-63. doi: 10.1016/j.virusres.2011.06.023. Epub 2011 Jul 6.
Viruses are most frequently discovered because they cause disease in organisms of importance to humans. To expand knowledge of plant-associated viruses beyond these narrow constraints, non-cultivated plants of the Tallgrass Prairie Preserve, Osage County, Oklahoma, USA were systematically surveyed for evidence of the presence of viruses. This report discusses viruses of the family Tombusviridae putatively identified by the survey. Evidence of two carmoviruses, a tombusvirus, a panicovirus and an unclassifiable tombusvirid was found. The complete genome sequence was obtained for putative TGP carmovirus 1 from the legume Lespedeza procumbens, and the virus was detected in several other plant species including the fern Pellaea atropurpurea. Phylogenetic analysis of the sequence and partial sequence of a related virus supported strongly the placement of these viruses in the genus Carmovirus. Polymorphisms in the sequences suggested existence of two populations of TGP carmovirus 1 in the study area and year-to-year variations in infection by TGP carmovirus 3.
病毒通常是因为它们会使对人类重要的生物体患病而被发现。为了将与植物相关的病毒的知识扩展到这些狭隘的限制之外，美国俄克拉荷马州奥塞奇县塔尔斯草甸保护区的非栽培植物被系统地调查，以寻找存在病毒的证据。本报告讨论了通过调查推测出的属于 Tmombusviridae 科的病毒。发现了两种 carmoviruses、一种 tombusvirus、一种 panicovirus 和一种无法分类的 tombusvirid。拟议的 TGP carmovirus 1 的全长基因组序列是从豆科植物蔓花生中获得的，该病毒在包括紫萁蕨在内的其他几种植物中被检测到。序列和相关病毒部分序列的系统发育分析强烈支持将这些病毒置于 Carmovirus 属中。序列中的多态性表明，在所研究的地区和年份，TGP carmovirus 1 存在两个种群，TGP carmovirus 3 的感染也存在年际变化。
