Cuhra Petr, Gabrovská Dana, Rysová Jana, Hanák Petr, Stumr Frantisek
Czech Agriculture and Food Inspection Authority, Za Opravnou 300/6, 150 06 Praha 5, Czech Republic.
J AOAC Int. 2011 Mar-Apr;94(2):605-10.
An interlaboratory study in 12 laboratories was performed to prove the validation of the ELISA method developed for the quantitative determination of mustard protein in foods. The ELISA kit used for this study is based on rabbit polyclonal antibody. This kit did not produce any false-positive results or cross-reactivity with in-house validation for a broad range of food matrixes with no detectable mustard protein. All participants obtained the Mustard ELISA kit with standard operational procedures, a list of samples, samples, and a protocol for recording test results. The study included 15 food samples and two spiked samples. Seven food matrix samples of zero mustard content and four samples with mustard declared as an ingredient showed mustard protein content lower than that of the first standard (0.42 mg/kg). Four samples with mustard declared as an ingredient revealed mustard protein content above 12.5 mg/kg (the highest standard). The statistical tests (Cochran, Dixon, and Mandel) and analysis of variance were used to evaluate the interlaboratory study results. Repeatability and reproducibility limits, as well as an LOQ (1.8 mg mustard proteins/kg) and LOD (0.5 mg mustard proteins/kg), for the kit were calculated.
在12个实验室进行了一项实验室间研究，以验证所开发的用于定量测定食品中芥子蛋白的ELISA方法。本研究使用的ELISA试剂盒基于兔多克隆抗体。该试剂盒在对多种不含可检测芥子蛋白的食品基质进行内部验证时，未产生任何假阳性结果或交叉反应。所有参与者均获得了带有标准操作程序、样品清单、样品以及测试结果记录方案的芥子ELISA试剂盒。该研究包括15个食品样品和两个加标样品。7个芥子含量为零的食品基质样品以及4个标明含有芥子成分的样品，其芥子蛋白含量低于第一个标准（0.42毫克/千克）。4个标明含有芥子成分的样品，其芥子蛋白含量高于12.5毫克/千克（最高标准）。使用统计检验（ Cochr an、Dixon和Mandel检验）和方差分析来评估实验室间研究结果。计算了该试剂盒的重复性和再现性限，以及定量限（1.8毫克芥子蛋白/千克）和检测限（0.5毫克芥子蛋白/千克）。
