Werner M, von Wasielewski R, Bernhards J, Georgii A
Pathologisches Institut, Medizinischen Hochschule Hannover, Federal Republic of Germany.
Virchows Arch A Pathol Anat Histopathol. 1990;416(5):411-6. doi: 10.1007/BF01605146.
A monoclonal antibody 7A9 was raised against the tumour-associated glycoprotein TAG-12 purified from T47-D breast carcinoma cells. In immunoblots from cytosol of T47-D cells and from sera of breast cancer patients, antibody 7A9 detects the high molecular weight mucin-like TAG-12 antigen. A series of paraffin sections of normal, benign and malignant mammary tissues have been studied with monoclonal antibody 7A9 and the immunoalkaline phosphatase method. In resting gland, proliferating gland and fibroadenoma ducts, reactivity of 7A9 was mainly restricted to luminal membranes of epithelial cells and secretions. 77/79 primary breast carcinomas including ductal, lobular and various other carcinoma types showed cytoplasmic and/or membrane-associated staining with 7A9 in most tumour cells. Metastases (31/31) from different sites were also positive. Strong immunoreactivity with single tumour cells was noted in cytological preparations from freshly resected breast cancer tissue. Thus, monoclonal antibody 7A9 seems to be very useful for the targeting of breast carcinoma cells.
一种单克隆抗体7A9是针对从T47 - D乳腺癌细胞中纯化的肿瘤相关糖蛋白TAG - 12制备的。在T47 - D细胞胞质溶胶和乳腺癌患者血清的免疫印迹中，抗体7A9可检测到高分子量的粘蛋白样TAG - 12抗原。利用单克隆抗体7A9和免疫碱性磷酸酶方法对一系列正常、良性和恶性乳腺组织的石蜡切片进行了研究。在静止腺体、增殖腺体和纤维腺瘤导管中，7A9的反应性主要局限于上皮细胞的腔膜和分泌物。77/79例原发性乳腺癌，包括导管癌、小叶癌和其他各种癌类型，大多数肿瘤细胞中7A9呈细胞质和/或膜相关染色。来自不同部位的转移灶（31/31）也呈阳性。在新鲜切除的乳腺癌组织的细胞学制备物中，观察到单个肿瘤细胞有强烈的免疫反应性。因此，单克隆抗体7A9似乎对靶向乳腺癌细胞非常有用。
