Nanogroup, University of Southampton, Southampton SO17 1BJ, United Kingdom.
Anal Chem. 2011 Jun 15;83(12):4814-21. doi: 10.1021/ac200463y. Epub 2011 May 24.
We present two microfluidic architectures (continuous flow and multiplexed stop flow) for miniaturized colorimetric nutrient sensors. These systems are compared with respect to the temporal response (for optimization of sampling rate) and reduction of reagent consumption. The continuous-flow system is capable of a sampling rate of 60 samples·h(-1), limited by Taylor dispersion. The novel multiplexed stop-flow (MSF) microsystem architecture is not limited by dispersion. A demonstration MSF system consisting of two stop-flow channels is presented. This requires 12.6 s to load each sample into a measurement channel and when scaled would be capable of a throughput of 285 h(-1) (with full color development). The MSF architecture is manufactured in PMMA/Viton/PMMA [where PMMA = poly(methyl methacrylate)], utilizes on-chip valving, and is scalable, thereby permitting sampling at much faster rates (subsecond). Either system is capable of remote deployment and continuous measurement of nutrient concentrations. The MSF system is particularly suited for applications requiring high temporal or spatial resolution; such as from moving vehicles.
我们提出了两种用于微型比色营养传感器的微流控架构（连续流和多路停止流）。这些系统在时间响应（用于优化采样率）和减少试剂消耗方面进行了比较。连续流系统的采样率可达 60 个样本·h(-1)，受到泰勒分散的限制。新型多路停止流（MSF）微系统架构不受分散限制。提出了一个由两个停止流通道组成的演示 MSF 系统。该系统需要 12.6 秒将每个样品加载到测量通道中，并且在扩展时将能够达到 285 h(-1)的吞吐量（完全显色）。MSF 架构采用 PMMA/Viton/PMMA（其中 PMMA = 聚甲基丙烯酸甲酯）制造，利用片上阀，并具有可扩展性，从而可以实现更快的采样率（亚秒级）。这两种系统都能够进行远程部署和连续的营养浓度测量。MSF 系统特别适用于需要高时间或空间分辨率的应用，例如从移动车辆中获取数据。
