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开发和鉴定 12 个多态性微卫星标记在美洲犬蜱(Dermacentor variabilis)中的应用。

Development and characterization of 12 polymorphic microsatellite loci in the American dog tick (Dermacentor variabilis).

机构信息

Department of Forestry and Natural Resources, Purdue University, Pfendler Building, West Lafayette, IN 47907, USA.

出版信息

Mol Ecol Resour. 2009 Jan;9(1):131-3. doi: 10.1111/j.1755-0998.2008.02313.x. Epub 2008 Oct 6.

DOI:10.1111/j.1755-0998.2008.02313.x
PMID:21564581
Abstract

Twelve polymorphic microsatellite loci were developed for the American dog tick (Dermacentor variabilis), an important vector of infectious diseases in humans and animals. Four multiplexed panels comprising the loci were developed and 45 ticks collected from two raccoons (Procyon lotor) were genotyped. The number of alleles per locus ranged from nine to 30, and single locus heterozygosities ranged from 0.18 to 0.93. Data generated using these markers will further our understanding of factors affecting gene flow in D. variabilis, thus helping to elucidate the transmission dynamics of diseases associated with this vector.

摘要

为美洲犬蜱(Dermacentor variabilis)开发了 12 个多态性微卫星基因座,该蜱是人类和动物传染病的重要传播媒介。开发了包含这 12 个基因座的四个多重基因座面板,并对从两只浣熊(Procyon lotor)身上采集的 45 只蜱进行了基因分型。每个基因座的等位基因数范围从 9 到 30,单个基因座的杂合度范围从 0.18 到 0.93。使用这些标记物生成的数据将进一步帮助我们了解影响 D. variabilis 基因流动的因素,从而有助于阐明与该媒介相关的疾病的传播动态。

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