Department of Biology/314, University of Nevada, Reno, Nevada 89557.
Mol Ecol Resour. 2009 Jan;9(1):134-6. doi: 10.1111/j.1755-0998.2008.02367.x. Epub 2008 Oct 6.
Thirteen newly developed tri- and tetranucleotide repeat microsatellite markers were developed for Lahontan cutthroat trout (Oncorhynchus clarki henshawi), a threatened subspecies endemic to the Lahontan hydrographic basin in the western USA. These loci are highly polymorphic with five to 30 alleles per locus and observed heterozygosities ranging from 0.4 to 0.7. Cross-species amplification of these markers was most successful in the closely related rainbow trout, Oncorhynchus mykiss, with only three loci amplifying in brown trout, Salmo trutta. Nonoverlapping allelic distributions for many of these loci among the six salmonid species screened suggest these markers may be useful for hybrid determination.
为拉洪坦鱒鱼（Oncorhynchus clarki henshawi）开发了 13 个新的三核苷酸和四核苷酸重复微卫星标记，这是一种在美国西部拉洪坦水系特有的受威胁亚种。这些位点具有高度多态性，每个位点有 5 到 30 个等位基因，观察到的杂合度从 0.4 到 0.7。这些标记在亲缘关系密切的虹鳟鱼（Oncorhynchus mykiss）中具有最高的跨物种扩增成功率，只有 3 个位点在褐鳟鱼（Salmo trutta）中扩增。在筛选的六种鲑鱼中，许多这些位点的等位基因分布没有重叠，这表明这些标记可能有助于杂种的鉴定。
