用于棕头鸦雀性别鉴定的新型引物及其在其他物种中的应用。

The novel primers for sex identification in the brown eared-pheasant and their application to other species.

机构信息

MOE Key Laboratory for Biodiversity Sciences and Ecological Engineering, College of Life Sciences, Beijing Normal University, Beijing 100875, China.

出版信息

Mol Ecol Resour. 2009 Jan;9(1):186-8. doi: 10.1111/j.1755-0998.2008.02177.x. Epub 2008 Oct 22.

DOI:10.1111/j.1755-0998.2008.02177.x

PMID:21564598

Abstract

We designed a pair of primers for sex identification in the brown eared-pheasant (Crossoptilon mantchuricum) based on the mechanism of PCR amplification of CHD fragments, and identified the number of products. The new primers were considered to have more sensitivity than P2/P8, and cross-species application indicated that they can also be used for sex identification in other species of Phasianidae and Passeriformes.

摘要

我们根据 CHD 片段 PCR 扩增的机制设计了一对引物，用于棕头雅雀（Crossoptilon mantchuricum）的性别鉴定，并确定了产物的数量。新的引物比 P2/P8 具有更高的灵敏度，种间应用表明它们也可用于雉科和雀形目其他物种的性别鉴定。