Seattle Aquarium, 1483 Alaskan Way, Pier 59, Seattle, WA 98101-2059, USA, Benaroya Research Institute at Virginia Mason, 1201 Ninth Ave., Seattle, WA 98101-2795, USA.
Mol Ecol Resour. 2009 May;9(3):978-81. doi: 10.1111/j.1755-0998.2009.02531.x. Epub 2009 Feb 5.
Novel sixgill shark (Hexanchus griseus) microsatellite loci were developed and tested on five shark species. A suite of microsatellite loci previously developed for lemon sharks (Negaprion brevirostris) was also tested. Data on 15 microsatellites are presented including primer sequences, number of alleles (a), observed (H(O) ) and expected heterozygosities (H(E) ), and F(IS) values for sixgill sharks (a = 10-69, H(O) = 0.24-1.00, H(E) = 0.76-0.96 and F(IS) = -0.21-0.60), sevengill sharks (Notorynchus cepedianus) (a = 6-40, H(O) = 0.20-0.73, H(E) = 0.59-0.94 and F(IS) = -0.47-0.58), Pacific spiny dogfish (Squalus acanthias) (a = 3-13, H(O) = 0.00-0.96, H(E) = 0.24-0.93 and F(IS) =-0.52-1.00), angle sharks (Squatina californica) (a = 1-4, H(O) = 0.00-1.00, H(E) = 0.60-1.00 and F(IS) =-1.00-0.25), and leopard sharks (Triakis semifasciata) (a = 3-16, H(O) = 0.20-1.00, H(E) = 0.53-0.92 and F(IS) = -0.57-1.00). A final suite of 14 microsatellites (13 developed from sixgill sharks and one from lemon sharks) were found to be polymorphic and conform to Hardy-Weinberg equilibrium within sixgill sharks.
开发并测试了 15 个微卫星标记，包括引物序列、等位基因数（a）、观测杂合度（H（O））、期望杂合度（H（E））和 F（IS）值，结果显示，六鳃鲨（Hexanchus griseus）的 a 值为 10-69，H（O）为 0.24-1.00，H（E）为 0.76-0.96，F（IS）为-0.21-0.60；七鳃鲨（Notorynchus cepedianus）的 a 值为 6-40，H（O）为 0.20-0.73，H（E）为 0.59-0.94，F（IS）为-0.47-0.58；短鳍灰鲭鲨（Squalus acanthias）的 a 值为 3-13，H（O）为 0.00-0.96，H（E）为 0.24-0.93，F（IS）为-0.52-1.00；尖吻鲭鲨（Squatina californica）的 a 值为 1-4，H（O）为 0.00-1.00，H（E）为 0.60-1.00，F（IS）为-1.00-0.25；豹纹鲨（Triakis semifasciata）的 a 值为 3-16，H（O）为 0.20-1.00，H（E）为 0.53-0.92，F（IS）为-0.57-1.00。最后，有 14 个微卫星标记（13 个来自六鳃鲨，1 个来自柠檬鲨）被发现是多态的，并且在六鳃鲨中符合哈迪-温伯格平衡。
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