UMR UPMC-CNRS-MNHN-IRD 7138, Département Systématique et Évolution, Muséum National d'Histoire Naturelle, Case Postale 26, 57 rue Cuvier, 75231 Paris Cedex 05, France.
Mol Ecol Resour. 2010 Jan;10(1):162-6. doi: 10.1111/j.1755-0998.2009.02732.x. Epub 2009 Jun 15.
The program phase is widely used for Bayesian inference of haplotypes from diploid genotypes; however, manually creating phase input files from sequence alignments is an error-prone and time-consuming process, especially when dealing with numerous variable sites and/or individuals. Here, a web tool called seqphase is presented that generates phase input files from fasta sequence alignments and converts phase output files back into fasta. During the production of the phase input file, several consistency checks are performed on the dataset and suitable command line options to be used for the actual phase data analysis are suggested. seqphase was written in perl and is freely accessible over the Internet at the address http://www.mnhn.fr/jfflot/seqphase.
程序相位广泛应用于从二倍体基因型中推断单倍型的贝叶斯推断；然而，从序列比对手动创建相位输入文件是一个容易出错且耗时的过程，尤其是在处理大量可变位点和/或个体时。这里，介绍了一个名为 seqphase 的网络工具，它可以从 fasta 序列比对生成相位输入文件，并将相位输出文件转换回 fasta。在生成相位输入文件的过程中，对数据集进行了多次一致性检查，并建议了用于实际相位数据分析的适当命令行选项。seqphase 是用 perl 编写的，可以通过互联网在地址 http://www.mnhn.fr/jfflot/seqphase 上免费访问。
