鉴定北美响尾蛇的单拷贝核 DNA 标记物。

Identification of single copy nuclear DNA markers for North American pit vipers.

机构信息

Department of Evolution, Ecology and Organismal Biology, Ohio State University, Columbus, OH 43210-1293, USA.

出版信息

Mol Ecol Resour. 2010 Jan;10(1):177-80. doi: 10.1111/j.1755-0998.2009.02707.x. Epub 2009 Apr 23.

DOI:10.1111/j.1755-0998.2009.02707.x

Abstract

We describe 18 single copy nuclear DNA loci (10 loci cloned from a Sistrurus catenatus catenatus genomic library and eight intron-based loci amplified using conserved primers) that detect sequence variation in species from all genera (Sistrurus, Agkistrodon and Crotalus) of North American pit viper snakes. These loci (mean size in bp ± SE: 433 ± 51) show large numbers of phylogenetically informative sites across species (mean + SE: 10.2 ± 1.5), but limited variation within subspecies suggesting that they will be most useful for multilocus species-level phylogenetic analyses in these snakes.

摘要

我们描述了 18 个单拷贝核 DNA 位点（10 个从 Sistrurus catenatus catenatus 基因组文库中克隆的位点和 8 个使用保守引物扩增的内含子），这些位点可检测来自北美的响尾蛇属（Sistrurus、Agkistrodon 和 Crotalus）所有属种的序列变异。这些位点（平均大小 bp±SE：433±51）在种间显示出大量具有系统发育信息的位点（平均+SE：10.2±1.5），但亚种内变异有限，这表明它们在这些蛇的多基因座种级系统发育分析中最有用。