U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA, 98115 USA Department of Biology, University of Washington, Seattle, WA 98195-1800, USA U.S. Bureau of Reclamation, Denver, CO, 80225, USA.
Mol Ecol Resour. 2010 Jan;10(1):190-2. doi: 10.1111/j.1755-0998.2009.02727.x. Epub 2009 Jun 15.
A 3-primer PCR system was developed to discriminate invasive zebra (Dreissena polymorpha) and quagga (Dreissena bugensis) mussel. The system is based on: 1) universal primers that amplifies a region of the nuclear 28s rDNA gene from both species and 2) a species-specific primer complementary to either zebra or quagga mussel. The species-specific primers bind to sequences between the binding sites for the universal primers resulting in the amplification of two products from the target species and one product from the nontarget species. Therefore, nontarget products are positive amplification controls. The 3-primer system accurately discriminated zebra and quagga mussels from seven geographically distinct populations.
开发了一种三引物 PCR 系统来区分入侵的斑马贝（Dreissena polymorpha）和斑背无齿蚌（Dreissena bugensis）。该系统基于：1）通用引物，可从两个物种的核 28s rDNA 基因扩增一个区域，以及 2）与斑马贝或斑背无齿蚌互补的物种特异性引物。物种特异性引物结合在通用引物的结合位点之间，导致从目标物种扩增出两个产物，从非目标物种扩增出一个产物。因此，非目标产物是阳性扩增对照。三引物系统能够准确地区分来自七个地理上不同种群的斑马贝和斑背无齿蚌。
