Department of Wildlife and Fisheries Sciences, Texas A&M University, 210 Nagle Hall, TAMU 2258, College Station, TX 77843, USA Department of Marine Biology, Texas A&M University at Galveston, 5007 Ave. U, Galveston, TX 77551, USA.
Mol Ecol Resour. 2010 Jan;10(1):193-6. doi: 10.1111/j.1755-0998.2009.02726.x. Epub 2009 Jun 15.
High-resolution melting analysis (HRMA) is a highly sensitive closed-tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase-A (ldh-A), myosin light chain-2 (mlc-2), acidic ribosomal phosphoprotein P0 (ARP) and calmodulin (CaM) in populations of swordfish, Xiphias gladius. Results indicate that HRMA is a powerful genotyping tool to study wild populations.
高分辨率熔解分析（HRMA）是一种高度敏感的闭管基因分型方法，主要用于临床研究。由于该方法快速、廉价且适用于高通量，我们决定研究其在群体研究中的适用性。我们使用小的扩增子和未标记的探针对箭鱼（Xiphias gladius）群体的核基因乳酸脱氢酶-A（ldh-A）、肌球蛋白轻链-2（mlc-2）、酸性核糖体磷蛋白 P0（ARP）和钙调蛋白（CaM）进行基因分型。结果表明，HRMA 是研究野生种群的强大基因分型工具。
