Laboratorio de Genética & Acuicultura, Universidad de Los Lagos, PO Box 933, Osorno, Chile Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, 9000 Ghent, Belgium.
Mol Ecol Resour. 2010 Jan;10(1):229-31. doi: 10.1111/j.1755-0998.2009.02744.x. Epub 2009 Jun 30.
The brine shrimp Artemia (Crustacea, Branchiopoda), a paradigmatic inhabitant of hypersaline lakes, has molecular features to survive under stressful conditions, such as the p26 heat shock protein. We report the RFLP fingerprinting pattern (four restriction enzymes) of a 217 bp fragment of exon2 of the Hsp26 gene in six Artemia franciscana and four Artemia persimilis populations, the most genetically divergent Artemia species co-occurring in latitudinal extremes of Chile. The species-specific RFLP pattern observed is a simple and cost-effective single-locus tool for species delimitation and experimental testing the habitat-induced isolation barrier between them.
卤虫（甲壳纲，鳃足亚纲）是一种典型的高盐湖泊栖息生物，具有在应激条件下生存的分子特征，如 p26 热休克蛋白。我们报告了 Hsp26 基因外显子 2 的 217bp 片段在六个卤虫 franciscana 和四个卤虫 persimilis 种群中的 RFLP 指纹图谱（四种限制酶），这是智利纬度极端地区共存的遗传差异最大的两种卤虫。观察到的种特异性 RFLP 模式是一种简单且具有成本效益的单基因座工具，可用于物种划分，并对它们之间由栖息地诱导的隔离障碍进行实验测试。
