Barash V, Erlich T, Bashan N
Department of Clinical Biochemistry, Hadassah University Hospital, Jerusalem, Israel.
Biochem Int. 1990;20(2):267-74.
An oxidative pathway of glucose-6-phosphate was found in the microsomal fraction of two extra-hepatic tissues: human placenta and pig kidney cortex. Oxidation activity in microsomes, measured by the formation of 14CO2 from [1-14C] glucose-6-phosphate, was observed only after Triton X-100 treatment and in the presence of methylene blue and NADP. Hexose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase were present in a latent form and required treatment with detergent for full activation. Our results suggest that these enzymes are located in the luminal space of placental and kidney microsomes, and that, as in the liver, they generate NADPH on the inner side of the endoplasmic reticulum when G6P and NADP are available.
在两种肝外组织（人胎盘和猪肾皮质）的微粒体部分中发现了葡萄糖-6-磷酸的氧化途径。通过[1-¹⁴C]葡萄糖-6-磷酸生成¹⁴CO₂来测定微粒体中的氧化活性，该活性仅在Triton X-100处理后以及在亚甲蓝和NADP存在的情况下才被观察到。己糖-6-磷酸脱氢酶和6-磷酸葡萄糖酸脱氢酶以潜伏形式存在，需要用去污剂处理才能完全激活。我们的结果表明，这些酶位于胎盘和肾微粒体的腔隙中，并且与肝脏一样，当有G6P和NADP时，它们在内质网内侧产生NADPH。
