Kardon Tamás, Senesi Silvia, Marcolongo Paola, Legeza Balázs, Bánhegyi Gábor, Mandl József, Fulceri Rosella, Benedetti Angelo
Semmelweis Egyetem Orvosi Vegytani, Molekuláris Biológiai és Patobiokémiai Intézete & MTA-SE Patobiokémiai Kutatócsoport, Budapest, Hungary.
FEBS Lett. 2008 Jun 11;582(13):1809-15. doi: 10.1016/j.febslet.2008.04.045. Epub 2008 May 8.
The present study demonstrates the expression of hexose-6-phosphate dehydrogenase and 11 beta-hydroxysteroid dehydrogenase type 1 in human neutrophils, and the presence and activity of these enzymes in the microsomal fraction of the cells. Their concerted action together with the previously described glucose-6-phosphate transporter is responsible for cortisone-cortisol interconversion detected in human neutrophils. Furthermore, the results suggest that luminal NADPH generation by the cortisol dehydrogenase activity of 11 beta-hydroxysteroid dehydrogenase type 1 prevents neutrophil apoptosis provoked by the inhibition of the glucose-6-phosphate transporter. In conclusion, the maintenance of the luminal NADPH pool is an important antiapoptotic factor in neutrophil granulocytes.
本研究证实了己糖-6-磷酸脱氢酶和11β-羟类固醇脱氢酶1型在人中性粒细胞中的表达，以及这些酶在细胞微粒体部分中的存在和活性。它们与先前描述的葡萄糖-6-磷酸转运体共同作用，导致了在人中性粒细胞中检测到的可的松-皮质醇相互转化。此外，结果表明，11β-羟类固醇脱氢酶1型的皮质醇脱氢酶活性产生的管腔NADPH可防止因葡萄糖-6-磷酸转运体抑制而引发的中性粒细胞凋亡。总之，管腔NADPH池的维持是中性粒细胞中的一个重要抗凋亡因子。
