Unité de Phylogénie et Génétique Moléculaires, Laboratoire de Systématique des Plantes et Biodiversité de l'Université de Genève, Conservatoire et Jardin Botaniques, Chemin de l'Impératrice 1, CP 60, CH 1292 Chambésy, Geneva, Switzerland.
Mol Ecol Resour. 2010 Jul;10(4):728-31. doi: 10.1111/j.1755-0998.2009.02816.x. Epub 2009 Dec 15.
Very divergent psbA-trnH chloroplast sequences were, for some Eryngiun alpinum individuals, repeatedly obtained and could not be attributed to contaminations nor to casual intraspecific variation. The design of external primers allowed the amplification of two different sequences for the same individuals. The divergent sequences were found to be more variable than their counterparts, to have a low GC content and to display a nonsynonymous substitution in psbA C-terminal region, all reasons that led us to hypothesize that they are paraloguous fragments transferred into the nucleus (NuPt). Quantitative polymerase chain reactions confirmed this hypothesis. Such NuPt might have severe implications in plant phylogeography and barcoding.
对于一些高山乳苣个体，我们多次获得了非常不同的 psbA-trnH 叶绿体序列，这些序列既不能归因于污染，也不能归因于偶然的种内变异。外部引物的设计允许对同一个体进行两种不同序列的扩增。研究发现，这些差异序列比其对应的序列具有更高的变异性，GC 含量较低，并在 psbA C 末端区域显示出非同义替换，所有这些原因使我们假设它们是转移到核内的（NuPt）旁系同源片段。定量聚合酶链反应证实了这一假设。这种 NuPt 可能对植物系统地理学和条形码产生严重影响。
