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四种ELISA试剂盒在检测感染率低的牛群的散装罐牛奶或浓缩乳糖血清中BLV抗体时的性能比较。

Comparison of performances of four ELISA kits in the detection of BLV antibodies in bulk tank milk or concentrated lactoserum from herds with low prevalence of infection.

作者信息

Eloit M, Kuzmak J, Dheiller M, Bénet J J, Toma B

机构信息

Ecole Vétérinaire, Maisons-Alfort, France.

出版信息

Biologicals. 1990 Jan;18(1):55-9. doi: 10.1016/1045-1056(90)90071-7.

Abstract

Performances of four ELISA kits in the detection of BLV antibodies in bulk tank milk was studied in 76 non-infected herds and 44 herds with low prevalence of BLV infection. None of the kits gave false positive results. On the other hand, there was an important variation in sensitivity. The kits with the highest sensitivity identified 43% of infected herds, which included 65% of infected cows. When concentrated lactoserum was tested, 59% of infected herds, which included 73% of infected cows, could be identified.

摘要

在76个未感染牛群和44个布氏白血病病毒(BLV)低感染率牛群中,研究了四种酶联免疫吸附测定(ELISA)试剂盒检测散装罐奶中BLV抗体的性能。没有一个试剂盒给出假阳性结果。另一方面,灵敏度存在重要差异。灵敏度最高的试剂盒识别出43%的感染牛群,其中包括65%的感染奶牛。当检测浓缩乳清时,可识别出59%的感染牛群,其中包括73%的感染奶牛。

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