Department of Biology, California State University, Northridge, 91330-8330, USA.
Acta Histochem. 2012 Feb;114(2):159-65. doi: 10.1016/j.acthis.2011.04.003. Epub 2011 May 11.
Peripheral neuropathy is a common complication of diabetes mediated by alterations of growth factors. Members of the neuropoietic cytokine family, which include IL-6, LIF, and CNTF among others, have been shown to be important regulators of peripheral nerves and the muscles that they innervate. To investigate their potential role in diabetic nerve and muscle, we studied the expression of the shared receptor subunits, LIFR and gp130 in a mouse model of streptozotocin (STZ)-induced diabetes. The results of Western blotting and densitometric analysis showed that both LIFR and gp130 protein expression were increased in diabetic sciatic nerve compared to control mice at early time points following STZ injection. In diabetic gastrocnemius muscle, LIFR and gp130 were increased from 3 days to 24 weeks following STZ injection. In contrast, both LIFR and gp130 protein expression were decreased in diabetic soleus muscle at 3-days post-injection. Our results suggest that hyperglycemia results in changes to nerve and muscle soon after the onset of diabetes and that cytokines may play a role in this process.
周围神经病变是糖尿病的一种常见并发症，由生长因子的改变介导。神经发生细胞因子家族的成员，包括白细胞介素 6 (IL-6)、白血病抑制因子 (LIF) 和睫状神经营养因子 (CNTF) 等，已被证明是周围神经和它们支配的肌肉的重要调节因子。为了研究它们在糖尿病神经和肌肉中的潜在作用，我们研究了在链脲佐菌素 (STZ) 诱导的糖尿病小鼠模型中，共同受体亚基 LIFR 和 gp130 的表达。Western blot 和密度分析的结果表明，与对照小鼠相比，STZ 注射后早期，糖尿病坐骨神经中 LIFR 和 gp130 蛋白表达增加。在糖尿病比目鱼肌中，LIFR 和 gp130 从 STZ 注射后 3 天到 24 周增加。相比之下，在 STZ 注射后 3 天，糖尿病跖肌中的 LIFR 和 gp130 蛋白表达降低。我们的结果表明，高血糖在糖尿病发病后不久就会导致神经和肌肉发生变化，细胞因子可能在这一过程中发挥作用。
