Berry K, Siegal G, Boyd J, Singh R, Fidler I
UNIV TEXAS,MD ANDERSON CANC CTR,DEPT CELL BIOL,BOX 173,1515 HOLCOMBE BLVD,HOUSTON,TX 77030. NIEHS,MOLEC CARCINOGENESIS LAB,RES TRIANGLE PK,NC 27709. UNIV ALABAMA,SCH MED,DEPT PATHOL,BIRMINGHAM,AL 35294. UNIV ALABAMA,SCH MED,DEPT CELL BIOL,BIRMINGHAM,AL 35294. UNIV ALABAMA,SCH MED,DEPT SURG,BIRMINGHAM,AL 35294.
Int J Oncol. 1994 Jun;4(6):1163-71. doi: 10.3892/ijo.4.6.1163.
We have developed an orthotopic model for human endometrial carcinoma in nude mice. The human serous papillary endometrial carcinoma cell line SPEC-2 was injected into the subcutis (ectopic site) or uterine wall (orthotopic site) of athymic mice. Tumors grew in both locations locally. However, only uterine wall tumors produced metastases in regional and distant lymph nodes and to the lungs and liver. Cell lines were established in culture from these uterine tumors and from lung and liver metastases, and then these cells were injected into the uteri of additional mice. The metastatic potential of the lines subsequently established from tumors growing in vivo was not significantly higher than the already highly metastatic parental culture cells. All SPEC-2 cell lines expressed high levels of both 72-kDa and 92-kDa collagenase type IV activity. mRNA for transforming growth factor-alpha, basic fibroblast growth factor, and epidermal growth factor-receptor was constant among the cell lines. These data support the concept that the orthotopic implantation of human endometrial carcinoma cells into the uteri of nude mice provides a valuable model for studying the biology of human endometrial adenocarcinoma.
我们已经在裸鼠中建立了人子宫内膜癌的原位模型。将人浆液性乳头状子宫内膜癌细胞系SPEC-2注射到无胸腺小鼠的皮下（异位部位）或子宫壁（原位部位）。肿瘤在这两个部位均局部生长。然而，只有子宫壁肿瘤在区域和远处淋巴结以及肺和肝脏发生转移。从这些子宫肿瘤以及肺和肝转移灶中培养建立细胞系，然后将这些细胞注射到其他小鼠的子宫中。随后从体内生长的肿瘤建立的细胞系的转移潜能并不显著高于已经具有高转移潜能的亲代培养细胞。所有SPEC-2细胞系均表达高水平的72-kDa和92-kDa IV型胶原酶活性。转化生长因子-α、碱性成纤维细胞生长因子和表皮生长因子受体的mRNA在各细胞系中保持恒定。这些数据支持这样的概念，即将人子宫内膜癌细胞原位植入裸鼠子宫为研究人子宫内膜腺癌的生物学特性提供了一个有价值的模型。
