Liu Xiao-Lin, Wang Zhao-Guo, Yang Ting-Ting, Yi Ying
Laboratory of Pathogenic Microorganism, Qingdao Municipal Center for Disease Control and Prevention, Qingdao 266033, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2011 Apr;32(4):382-4.
To study the molecular epidemiology of human enterovirus 71 (EV71) isolated from patients with hand-foot-mouth disease (HFMD) in Qingdao region between 2007 and 2009.
Throat swabs of HFMD were detected for total enterovirus (EV), EV71 and CA16 by fluorescence reverse transcription-polymerase chain reaction (RT-PCR). The VP1 region was amplified from positive EV71 specimens and 51 nucleotide sequences of VP1 gene were successfully sequenced. EV71 genotypes were characterized by phylogenetic analysis.
51 Qingdao EV71 strains identified between 2007 and 2009 belonged to C4a cluster of subgenotype C4 and 5.3% nucleotide divergence and 1.7% amino acid divergence were found among them. The strains in 2008 and 2009 were respectively divided into several lineages on phylogenetic trees but there was a main lineage (namely prominent strain) every year. The Qingdao prominent strains identified in 2008 had high identities with the Linyi prominent strains in 2007. The Qingdao prominent strains in 2009 and the secondary prominent strains in 2008 had high identities with the Fuyang prominent strains in 2008. Two Qingdao strains in 2007 had high identities with the Linyi popular strains in 2007.
Data showed that several EV71 transmission chains were co-circulating in Qingdao between 2008 and 2009. The main transmission chain in 2008 became the minor one but the minor transmission chain in 2008 was transferred into the main transmission chain in 2009.
研究2007年至2009年期间从青岛地区手足口病（HFMD）患者中分离出的人肠道病毒71型（EV71）的分子流行病学。
采用荧光逆转录-聚合酶链反应（RT-PCR）检测手足口病患者的咽拭子中的总肠道病毒（EV）、EV71和CA16。从阳性EV71标本中扩增VP1区域，并成功测序51个VP1基因的核苷酸序列。通过系统发育分析确定EV71基因型。
2007年至2009年期间鉴定出的51株青岛EV71毒株属于C4亚型的C4a簇，它们之间存在5.3%的核苷酸差异和1.7%的氨基酸差异。2008年和2009年的毒株在系统发育树上分别分为几个谱系，但每年都有一个主要谱系（即优势毒株）。2008年鉴定出的青岛优势毒株与2007年的临沂优势毒株具有高度同源性。2009年的青岛优势毒株和2008年的次要优势毒株与2008年的阜阳优势毒株具有高度同源性。2007年的两株青岛毒株与2007年的临沂流行毒株具有高度同源性。
数据显示，2008年至2009年期间青岛有多个EV71传播链同时流行。2008年的主要传播链成为次要传播链，而2008年的次要传播链在2009年转变为主要传播链。
