Takeno S
Department of Otolaryngology, Hiroshima University School of Medicine.
Nihon Jibiinkoka Gakkai Kaiho. 1990 Jan;93(1):33-9. doi: 10.3950/jibiinkoka.93.33.
Primary cell culture system from middle-ear epithelium of the guinea pig was established in defined condition. Mucosal cells were dispersed with enzymatic procedure and over 90% of the cell viability was obtained. Collagen gel and fibronectin coated Thermanox plate were used as culture substrates, and cultured cells on both materials formed confluent epithelial linings. Histochemical localization of succinate dehydrogenase, cytochrome oxidase and adenosine triphosphatase in mitochondria were examined. Cultured ciliated cells and some non-ciliated cells with numerous microvilli showed strong activities of succinate dehydrogenase and cytochrome oxidase. Also in vivo, normal ciliated epithelium near the eustachian tube in the middle-ear cavity of the guinea pig revealed strong mitochondrial metabolic activities. We concluded that this system would be useful for the study of cellular multiplication and differentiation systems of the middle-ear epithelium.
在特定条件下建立了豚鼠中耳上皮的原代细胞培养系统。采用酶解法分散黏膜细胞，细胞活力超过90%。用胶原蛋白凝胶和纤连蛋白包被的瑟曼诺克斯培养板作为培养底物，两种材料上培养的细胞均形成汇合的上皮衬里。检测了线粒体中琥珀酸脱氢酶、细胞色素氧化酶和三磷酸腺苷酶的组织化学定位。培养的纤毛细胞和一些具有大量微绒毛的非纤毛细胞显示出较强的琥珀酸脱氢酶和细胞色素氧化酶活性。在体内，豚鼠中耳腔咽鼓管附近的正常纤毛上皮也显示出较强的线粒体代谢活性。我们得出结论，该系统将有助于研究中耳上皮的细胞增殖和分化系统。
