Chunxiao Wang, Jingjing Liu, Yire Xiao, Jingning Lu, Kai Kang, Liang Shen, Yi Lu, Rasco Barbara
Laboratory of Minigene Pharmacy, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.
Protein Expr Purif. 2011 Sep;79(1):156-63. doi: 10.1016/j.pep.2011.04.017. Epub 2011 May 4.
A recombinant human parathyroid hormone fragment, Pro-Pro-[Arg(11)]hPTH(1-34)-Pro-Pro (MW=4550 Da), was developed by substituting Arg for Leu at position 11 and adding Pro-Pro at the carboxyl terminus. Following a single injection (0.5-13.5 μg/bird) of the rhPTH fragment, the serum calcium level in chickens increased 12-42% (P<0.05) after 1h as determined by the Parson's Chicken Assay. The functional activity of Pro-Pro-[Arg(11)]hPTH(1-34)-Pro-Pro may be due to removal of the N-terminus Pro-Pro- by X-prolyl dipeptidyl peptidase IV (DPPIV) in vivo, increasing its activity compared to Pro-Pro-hPTH(1-34). This artificial rhPTH fragment could be used to increase calcium mobilization and potentially improve bone health.
一种重组人甲状旁腺激素片段，即Pro-Pro-[Arg(11)]hPTH(1-34)-Pro-Pro（分子量=4550道尔顿），是通过将第11位的亮氨酸替换为精氨酸并在羧基末端添加Pro-Pro而研发出来的。单次注射（0.5 - 13.5μg/只鸡）该重组人甲状旁腺激素片段后，采用帕森斯鸡检测法测定，鸡血清钙水平在1小时后升高了12 - 42%（P<0.05）。Pro-Pro-[Arg(11)]hPTH(1-34)-Pro-Pro的功能活性可能是由于体内X-脯氨酰二肽基肽酶IV（DPPIV）去除了N端的Pro-Pro-，与Pro-Pro-hPTH(1-34)相比，其活性有所增加。这种人工重组人甲状旁腺激素片段可用于增加钙动员，并有可能改善骨骼健康。
