Mackow E R, Vo P T, Broome R, Bass D, Greenberg H B
Department of Microbiology and Immunology, Stanford University, California 94305.
J Virol. 1990 Apr;64(4):1698-703. doi: 10.1128/JVI.64.4.1698-1703.1990.
A baculovirus-expressed VP4 protein derived from the simian rhesus rotavirus (RRV) was used to parenterally immunize murine dams. VP4-immunized dams developed high levels of neutralizing antibodies against RRV and low levels of cross-reactive neutralizing antibodies against human strains Wa, ST3, and S2 and animal strains SA-11, NCDV, and Eb. Newborn mice suckled on VP4-immunized dams were protected against a virulent challenge dose of the simian strain RRV and against murine rotavirus Eb. The cross-reactive nature of the serum-neutralizing response generated by VP4 immunization and the protective efficacy of the immunization suggest that recombinant-expressed VP4 proteins should be considered as viable vaccine candidates.
一种源自恒河猴轮状病毒（RRV）的杆状病毒表达的VP4蛋白被用于对母鼠进行肠道外免疫。用VP4免疫的母鼠产生了高水平的针对RRV的中和抗体，以及低水平的针对人毒株Wa、ST3和S2以及动物毒株SA-11、NCDV和Eb的交叉反应性中和抗体。吃了用VP4免疫的母鼠乳汁的新生小鼠对恒河猴毒株RRV的强毒攻击剂量以及对鼠轮状病毒Eb具有抵抗力。VP4免疫产生的血清中和反应的交叉反应性质以及免疫的保护效果表明，重组表达的VP4蛋白应被视为可行的疫苗候选物。
