School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510070, China.
Bioresour Technol. 2011 Jul;102(14):7093-8. doi: 10.1016/j.biortech.2011.04.073. Epub 2011 Apr 28.
Biofilms formation capacities of Shewanella species in microbial fuel cells (MFCs) and their roles in current generation have been documented to be species-dependent. Understandings of the biofilms growth and metabolism are essential to optimize the current generation of MFCs. Shewanella decolorationis S12 was used in both closed-circuit and open-circuit MFCs in this study. The anodic S. decolorationis S12 biofilms could generate fivefold more current than the planktonic cells, playing a dominant role in current generation. Anodic biofilms viability was sustained at 98 ± 1.2% in closed-circuit while biofilms viability in open-circuit decreased to 72 ± 7% within 96 h. The unviable domain in open-circuit MFCs biofilms majorly located at the inner layer of biofilm. The decreased biofilms viability in open-circuit MFCs could be recovered by switching into closed-circuit, indicating that the current-generating anode in MFCs could serve as a favorable electron acceptor and provide sufficient energy to support cell growth and metabolism inside biofilms.
已有文献记录表明，希瓦氏菌属（Shewanella）在微生物燃料电池（MFC）中形成生物膜的能力及其在当前电流产生中的作用取决于物种。了解生物膜的生长和代谢对于优化 MFC 的当前电流产生至关重要。在本研究中，使用了脱色希瓦氏菌（Shewanella decolorationis）S12 同时在闭路和开路 MFC 中进行实验。与浮游细胞相比，阳极上的希瓦氏菌（Shewanella decolorationis）S12 生物膜能够产生五倍的电流，在电流产生中起着主导作用。闭路中阳极生物膜的存活率维持在 98±1.2%，而在 96 小时内，开路中生物膜的存活率下降到 72±7%。开路 MFC 生物膜中无活力区域主要位于生物膜的内层。开路 MFC 中生物膜的存活率降低可以通过切换到闭路来恢复，这表明 MFC 中的电流产生阳极可以作为一种有利的电子受体，并提供足够的能量来支持生物膜内细胞的生长和代谢。
