Nishi I, Sunada A, Toyokawa M, Asari S, Iwatani Y
Laboratory for Clinical Investigation, Osaka University Hospital, 2-15 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Chemother. 2011 Apr;23(2):102-6. doi: 10.1179/joc.2011.23.2.102.
We aimed to evaluate the efficacy of a combination of amphotericin B (AMB) and micafungin (MCFG) against 25 clinical isolates of Aspergillus species in vitro. We examined fungal growth in the presence of these drugs using a checkerboard method with the tetrazolium salt: 2,3-bis (2- methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxyanilide inner salt (XTT) to determine the efficacy of an AMB/MCFG combination in inhibition of filamentous fungal growth, evaluated based on 50% reduction of metabolic activity. The fractional inhibitory concentration index showed that the drugs synergistically inhibited 36% of the isolates. Activity was judged as indifferent for 64% isolates; antagonistic interaction was not detected. The AMB/MCFG combination was more effective than AMB alone when sub-inhibitory concentrations of AMB were used. This report demonstrates the efficacy of AMB/MCFG combination for inhibiting the growth of Aspergillus species in vitro, warranting the extension of such studies to animal models.
我们旨在评估两性霉素B（AMB）和米卡芬净（MCFG）联合用药对25株临床分离曲霉菌种的体外抗菌效果。我们采用棋盘法，在有这些药物存在的情况下，使用四氮唑盐：2,3-双（2-甲氧基-4-硝基-5-磺基苯基）-2H-四氮唑-5-羧基苯胺内盐（XTT）检测真菌生长情况，以确定AMB/MCFG联合用药对丝状真菌生长的抑制效果，基于代谢活性降低50%进行评估。部分抑菌浓度指数显示，这些药物对36%的分离株有协同抑制作用。64%的分离株活性被判定为无差异；未检测到拮抗相互作用。当使用亚抑菌浓度的AMB时，AMB/MCFG联合用药比单独使用AMB更有效。本报告证明了AMB/MCFG联合用药在体外抑制曲霉菌种生长的效果，值得将此类研究扩展至动物模型。
