Jilin Province Animal Embryo Engineering Key Laboratory, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, 130062, China.
J Anim Sci. 2011 Oct;89(10):3099-106. doi: 10.2527/jas.2010-3827. Epub 2011 May 13.
Myostatin is expressed in skeletal muscle tissue where it functions to suppress myoblast proliferation and myofiber hypertrophy. Recently, myostatin was detected in the tendon, mammary gland, and adipose tissue of mice. We sought to determine whether myostatin is expressed in the liver, spleen, lung, and kidney of pigs. Real-time PCR and Western blots demonstrated that myostatin, follistatin, decorin, and activin receptor IIB (ActRIIB) mRNA and proteins were expressed in skeletal muscle, heart muscle, and adipose tissue, and also in liver, spleen, lung, kidney, and cultured fibroblasts. The relative abundance of myostatin was closely related to follistatin and decorin in porcine tissues. Immunohistochemical analysis further demonstrated the presence of myostatin, follistatin, and decorin in the skeletal muscle, adipose tissue, heart muscle, liver, spleen, lung, and kidney of pigs. These results suggest that myostatin could be associated with certain functions of the internal organs, such as energy metabolism or fibrosis. We conclude that myostatin is a factor broadly expressed in the internal organs and muscle tissues of pigs.
肌肉生长抑制素在骨骼肌组织中表达，其功能是抑制成肌细胞增殖和肌纤维肥大。最近，在小鼠的肌腱、乳腺和脂肪组织中检测到肌肉生长抑制素。我们试图确定肌肉生长抑制素是否在猪的肝脏、脾脏、肺和肾脏中表达。实时 PCR 和 Western blot 表明，肌肉生长抑制素、卵泡抑素、核心蛋白聚糖和激活素受体 IIB（ActRIIB）mRNA 和蛋白质在骨骼肌、心肌和脂肪组织中表达，也在肝脏、脾脏、肺、肾脏和培养的成纤维细胞中表达。肌肉生长抑制素的相对丰度与猪组织中的卵泡抑素和核心蛋白聚糖密切相关。免疫组织化学分析进一步表明，肌肉生长抑制素、卵泡抑素和核心蛋白聚糖存在于猪的骨骼肌、脂肪组织、心肌、肝脏、脾脏、肺和肾脏中。这些结果表明，肌肉生长抑制素可能与某些内部器官的功能有关，如能量代谢或纤维化。我们得出结论，肌肉生长抑制素是一种在猪的内部器官和肌肉组织中广泛表达的因子。
