抗阿霉素（dxr）单克隆抗体——一些特性及在阿霉素免疫测定中的应用

Monoclonal-antibody against Doxorubicin (dxr) - some characteristics and utilization for dxr-immunoassay.

作者信息

Ohkawa K, Hatano T, Takada K, Tsukada Y, Matsuda M

机构信息

SRL,DIV BIOMED,HACHIOJI,TOKYO 192,JAPAN.

出版信息

Int J Oncol. 1993 Jul;3(1):43-6. doi: 10.3892/ijo.3.1.43.

DOI:10.3892/ijo.3.1.43

PMID:21573323

Abstract

To establish a rapid, specific, sensitive and simple assay method for doxorubicin (DXR) in body fluid, monoclonal antibodies (MAbs) against DXR were generated by immunizing mice with keyhole limpet hemocyanin-DXR conjugate, cell fusion, and a one step, time saving screening ELISA method using aminoplate-coupled DXR via a glutaraldehyde bridge as solid phase antigen. Inhibition ELISA for DXR-immunoassay was established using anti-DXR MAb of the best producer (2E9) and aminoplate-coupled DXR as antigen and DXR ranging from 50 pg to 50 ng in the body fluid or in the cell extract could be detected. MAb 2E9 cross-reacted to various degrees to anthracycline compounds, such as some DXR analogues and derivatives, but did not recognize anthracene and anthraquinone structures.

摘要

为建立一种快速、特异、灵敏且简便的体液中阿霉素（DXR）检测方法，用钥孔戚血蓝蛋白-阿霉素偶联物免疫小鼠，经细胞融合，采用通过戊二醛桥将氨基板偶联阿霉素作为固相抗原的一步省时筛选ELISA法，制备了抗阿霉素单克隆抗体（MAbs）。使用最佳产生者的抗DXR单克隆抗体（2E9）和氨基板偶联DXR作为抗原，建立了用于DXR免疫分析的抑制ELISA法，可检测体液或细胞提取物中50 pg至50 ng范围内的DXR。单克隆抗体2E9与各种蒽环类化合物有不同程度的交叉反应，如一些DXR类似物和衍生物，但不识别蒽和蒽醌结构。