Volling P, Jungehulsing M, Stutzer H, Diehl V, Tesch H
UNIV COLOGNE,INST DOKUMENTAT & STAT,W-5000 COLOGNE 41,GERMANY. UNIV COLOGNE,MED 1 KLIN,W-5000 COLOGNE 41,GERMANY.
Int J Oncol. 1993 Oct;3(4):671-7. doi: 10.3892/ijo.3.4.671.
We have analysed DNA and RNA from 40 untreated head and neck cancers for amplified and overexpressed proto-oncogenes by Southern and Northern blot hybridisation. Coamplification but no expression of the bcl-1 and the hst genes was found in 12.5% of the patients. Furthermore, amplifications of c-myc were found in 10%, of Ha-ras and c-erbB-2 in 5%. c-erbB-2 amplification was accompanied by gene expression but no overexpression. Correlating our results with clinicopathological data of the patients amplifications were only found in stage III and IV disease (p=0.0164). No correlation was found between amplification and primary tumour site, histopathological differentiation of the tumours, response to induction chemotherapy, or survival. Our results indicate that oncogene amplification in advanced SCCHN reflects more the general genomic instability of advanced tumours than be a reason for tumour growth.
我们通过Southern和Northern印迹杂交分析了40例未经治疗的头颈癌的DNA和RNA，以检测原癌基因的扩增和过表达情况。在12.5%的患者中发现了bcl-1和hst基因的共扩增，但无表达。此外，10%的患者发现c-myc扩增，5%的患者发现Ha-ras和c-erbB-2扩增。c-erbB-2扩增伴有基因表达，但无过表达。将我们的结果与患者的临床病理数据相关联，发现扩增仅出现在III期和IV期疾病中（p=0.0164）。未发现扩增与原发肿瘤部位、肿瘤的组织病理学分化、诱导化疗反应或生存率之间存在相关性。我们的结果表明，晚期头颈部鳞状细胞癌中的癌基因扩增更多地反映了晚期肿瘤的一般基因组不稳定性，而非肿瘤生长的原因。
