Department of Preventive Dentistry, Hospital of Stomatology, Fujian Medical University, 246 Yangqiao Zhong Road, Fuzhou 350002, PR China.
Oncol Rep. 2011 Jul;26(1):101-8. doi: 10.3892/or.2011.1286. Epub 2011 Apr 28.
Cadherins are found in almost all living organisms. In addition to their role in the formation and maintenance of normal tissue architecture, cadherins seem to play a crucial role in the cell-cell interactions of cancer cells in tumorigenesis, invasion and metastasis. The aim of the present study was to identify the role of CDH12 in the invasion and metastasis of salivary adenoid cystic carcinoma (SACC). Real-time PCR results showed that CDH12 is abnormally expressed in the highly metastatic SACC cell line ACC-M, compared to ACC-2, a SACC cell line with low metastatic ability. CDH12 expression was significantly higher in clinical samples with metastasis and recurrence than in those without metastasis and recurrence (P<0.05), as demonstrated by immunohistochemical analysis. Overexpression of the CDH12 protein in ACC-M cells infected with an adenovirus vector containing CDH12 enhanced the invasive and migratory ability of ACC-M cells in vitro compared to ACC-M cells infected with empty vector. Likewise, knockdown of CDH12 by small interfering RNA efficiently inhibited the invasion and migration of ACC-M cells in vitro. These results indicate that CDH12 may play an important role in the invasion and metastasis of SACC.
钙黏蛋白几乎存在于所有生物中。除了在正常组织架构的形成和维持中发挥作用外，钙黏蛋白似乎在肿瘤发生、侵袭和转移过程中癌细胞的细胞-细胞相互作用中发挥着至关重要的作用。本研究旨在确定 CDH12 在唾液腺腺样囊性癌（SACC）侵袭和转移中的作用。实时 PCR 结果显示，与低转移能力的 SACC 细胞系 ACC-2 相比，高度转移的 SACC 细胞系 ACC-M 中 CDH12 异常表达。免疫组织化学分析显示，具有转移和复发的临床样本中 CDH12 的表达明显高于无转移和复发的样本（P<0.05）。用含有 CDH12 的腺病毒载体感染的 ACC-M 细胞中 CDH12 蛋白的过表达增强了 ACC-M 细胞在体外的侵袭和迁移能力，而空载体感染的 ACC-M 细胞则没有。同样，通过小干扰 RNA 敲低 CDH12 可有效抑制 ACC-M 细胞在体外的侵袭和迁移。这些结果表明，CDH12 可能在 SACC 的侵袭和转移中发挥重要作用。
