Kitamura K, Mizuno Y, Hatayama I, Sato K, Kikuchi K
HOKKAIDO UNIV,INST IMMUNOL SCI,BIOCHEM SECT,KITA 15,NISHI 7,KITA KU,SAPPORO,HOKKAIDO 060,JAPAN. HIROSAKI UNIV,DEPT BIOCHEM 2,HIROSAKI,AOMORI 036,JAPAN.
Int J Oncol. 1993 Feb;2(2):237-40. doi: 10.3892/ijo.2.2.237.
We have examined the mRNA levels for the catalytic subunits of three phosphoseryl/phosphothreonyl protein phosphatases PP1alpha, PP2Aalpha and PP2C by Northern blot analysis in the three primary hepatomas and the three poorly differentiated ascites hepatomas. The mRNA levels were compared with those in regenerating rat livers. In regenerating rat livers, the mRNA levels of PP1alpha, PP2Aalpha and PP2C at 24 h after partial hepatectomy were elevated 5, 14 and 10 times as compared to those of the control livers. But in the three primary hepatomas induced by 3'-methyl-4-dimethyl-aminoazobenzene(3'-Me-DAB), 2-acetylaminofluorene(2-AAF), and induced by the Solt-Farber model, the mRNA levels of PP1alpha, PP2Aalpha and PP2C were almost the same as those of the control livers. On the other hand, in the three poorly differentiated ascites hepatomas, AH13, AH66F and AH130, PP1alpha mRNA levels were 10 times higher than that of the control livers. In these ascites hepatomas, PP2Aalpha mRNA levels were distinctively lower than that of the control livers. PP2C mRNA levels were the same in AH13 and lower in AH66F and AH130 compared to the controls. From these results, it was demonstrated that PP1alpha mRNA levels were selectively increased in poorly differentiated ascites hepatoma cells.
我们通过Northern印迹分析检测了三种磷酸丝氨酸/磷酸苏氨酸蛋白磷酸酶PP1α、PP2Aα和PP2C的催化亚基在三种原发性肝癌和三种低分化腹水肝癌中的mRNA水平。将这些mRNA水平与再生大鼠肝脏中的水平进行了比较。在再生大鼠肝脏中，部分肝切除术后24小时，PP1α、PP2Aα和PP2C的mRNA水平与对照肝脏相比分别升高了5倍、14倍和10倍。但是在由3'-甲基-4-二甲基氨基偶氮苯（3'-Me-DAB）、2-乙酰氨基芴（2-AAF）诱导的三种原发性肝癌以及由索尔特-法伯模型诱导的原发性肝癌中，PP1α、PP2Aα和PP2C的mRNA水平与对照肝脏几乎相同。另一方面，在三种低分化腹水肝癌AH13、AH66F和AH130中，PP1α的mRNA水平比对照肝脏高10倍。在这些腹水肝癌中，PP2Aα的mRNA水平明显低于对照肝脏。PP2C的mRNA水平在AH13中与对照相同，在AH66F和AH130中低于对照。从这些结果可以证明，PP1α的mRNA水平在低分化腹水肝癌细胞中选择性增加。
