Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe 657-8501, Japan.
Appl Microbiol Biotechnol. 2011 Aug;91(4):1001-6. doi: 10.1007/s00253-011-3196-4. Epub 2011 May 14.
We developed a novel enzymatic glutathione (GSH) production system using Saccharomyces cerevisiae as a whole-cell biocatalyst, and improved its GSH productivity by metabolic engineering. We demonstrated that the metabolic engineering of GSH pathway and ATP regeneration can significantly improve GSH productivity by up to 1.7-fold higher compared with the parental strain, respectively. Furthermore, the combination of both improvements in GSH pathway and ATP regeneration is more effective (2.6-fold) than either improvement individually for GSH enzymatic production using yeast. The improved whole-cell biocatalyst indicates its great potential for applications to other kinds of ATP-dependent bioproduction.
我们开发了一种新型的利用酿酒酵母作为全细胞生物催化剂的酶法谷胱甘肽 (GSH) 生产系统，并通过代谢工程提高了其 GSH 生产能力。我们证明，通过代谢工程分别对 GSH 途径和 ATP 再生的改造可以使 GSH 生产力分别比原始菌株提高 1.7 倍和 1.7 倍。此外，与分别对 GSH 途径和 ATP 再生的改进相比，两者的结合对于使用酵母进行酶法生产 GSH 更为有效（提高 2.6 倍）。经过改进的全细胞生物催化剂表明，它在其他类型的依赖于 ATP 的生物生产中具有很大的应用潜力。
