Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an, China.
Anal Bioanal Chem. 2011 Jul;401(1):229-35. doi: 10.1007/s00216-011-5071-7. Epub 2011 May 14.
Ag(+) has been regarded as an inert chemiluminescent oxidant. In this work, it was found that in the presence of silver nanoparticles (AgNPs), AgNO(3) could react with luminol to produce strong chemiluminescence (CL). The AgNPs with smaller size could initiate stronger CL emission. To investigate the CL mechanism of the AgNPs-luminol-AgNO(3) system, the UV-visible spectra and the CL spectrum of the CL system were obtained. The CL reaction mechanism involving catalysis was proposed. Compared with the reported nanoparticles-luminol-H(2)O(2) CL system, the AgNPs-luminol-AgNO(3) CL system has the advantages of low background and good stability. Moreover, the new CL system was used in immunoassay for IgG.
Ag(+) 一直被认为是一种惰性化学发光氧化剂。在这项工作中，我们发现，在银纳米粒子（AgNPs）的存在下，AgNO(3) 可以与鲁米诺反应产生强烈的化学发光（CL）。尺寸较小的 AgNPs 可以引发更强的 CL 发射。为了研究 AgNPs-鲁米诺-AgNO(3) 体系的 CL 机制，我们获得了 CL 体系的紫外-可见光谱和 CL 光谱。提出了涉及催化的 CL 反应机制。与已报道的纳米粒子-鲁米诺-H(2)O(2) CL 体系相比，AgNPs-鲁米诺-AgNO(3) CL 体系具有背景低、稳定性好的优点。此外，新的 CL 体系还被用于 IgG 的免疫测定。
