Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia.
Anal Bioanal Chem. 2011 Jul;401(1):381-6. doi: 10.1007/s00216-011-5070-8. Epub 2011 May 15.
The bright chemiluminescence has been observed in the system: Co(2+)/hydrogen peroxide/lucigenin. The chemiluminescence intensity was directly proportional to either cobalt, hydrogen peroxide, or lucigenin concentrations. A procedure of determination of superoxide dismutase (SOD) activity by the chemiluminescence method in the cobalt-hydrogen peroxide-lucigenin system at pH 8.5 is suggested. A linear dependence was established between a relative chemiluminescence intensity and SOD concentration in the range of SOD concentrations between 0 and 4.5 nM, c (1/2) = 0.8 nM. The determination of SOD activity was performed in several tissue samples (rat plasma, erythrocyte hemolysate, and liver mitochondria). A technique of tissue sample preparation with the use of thermal inactivation of interfering proteins at 60 °C was used. The method was successfully applied for comparison of the efficiency of SOD mimetics.
在 Co(2+)/过氧化氢/荧光素体系中观察到了明亮的化学发光。化学发光强度与钴、过氧化氢或荧光素的浓度成正比。本文提出了一种在 pH 8.5 的钴-过氧化氢-荧光素体系中通过化学发光法测定超氧化物歧化酶（SOD）活性的方法。在 0 到 4.5 nM 的 SOD 浓度范围内，相对化学发光强度与 SOD 浓度之间建立了线性关系，c(1/2)=0.8 nM。在几种组织样品（大鼠血浆、红细胞溶血和肝线粒体）中进行了 SOD 活性的测定。使用在 60°C 下热失活干扰蛋白的组织样品制备技术。该方法成功地应用于比较 SOD 模拟物的效率。
