Doughty Michael J
Department of Vision Sciences, Cowcaddens Road, Glasgow-Caledonian University, Glasgow G4 OBA, Scotland.
Diagn Cytopathol. 2011 Jun;39(6):409-23. doi: 10.1002/dc.21404. Epub 2010 May 14.
The main aim of this work is to present a special method for conjunctival impression cytology (CIC) and objective measures of measure cell and nucleus dimensions. CIC using Millicell® Biopore filters was obtained from 50 Caucasian adults from the nasal bulbar conjunctiva. Giemsa-stained images of cells in a monolayer were photographed with a scale bar and projected at 1,500× to generate an overlay. Measures were made of both the long (L) and short (S) dimensions of the cells and the longest dimension of the nucleus (LN). The selected images comprised sets of 10 from different individuals, which had been assigned grades 0, 1, 2, or 3 and an additional grade 0.5. L ranged from 18.4 to 65.2 μm, S from 13.1 to 47.1 μm, to give average cell shape values (L:S ratio) of between 1.259 and 1.890. L and S were smallest for grade 0 cells (group mean 19.8 ± 1.1 and 14.8 ± 1.2 μm) and largest for grade 3 cells (group 51.2 ± 7.2 and 37.2 ± 4.7 μm), with no difference in L:S ratios across the cell grades. Longest dimension of the nucleus (NL) values ranged from 8.7 to 14.5 μm, with group mean values of 9.8 ± 0.6 μm for grade 0 cells and 12.9 ± 1.2 μm for grade 3 cells. NL was nonlinearly associated with L. Measures of conjunctival cell and nucleus dimensions can be used to objectively distinguish between cells assigned different grades of squamous metaplasia, with slight nucleus enlargement being the earliest detectable change (at grade 0.5).
这项工作的主要目的是介绍一种用于结膜印迹细胞学（CIC）的特殊方法以及测量细胞和细胞核尺寸的客观指标。使用Millicell®生物孔滤膜获得的CIC样本来自50名白种成年人的鼻侧球结膜。用带有比例尺的相机拍摄单层细胞的吉姆萨染色图像，并以1500倍投影以生成叠加图。对细胞的长（L）和短（S）尺寸以及细胞核的最长尺寸（LN）进行测量。所选图像包括来自不同个体的10组图像，这些图像被分为0、1、2或3级，另外还有0.5级。L的范围为18.4至65.2μm，S的范围为13.1至47.1μm，细胞形状平均值（L:S比值）在1.259至1.890之间。0级细胞的L和S最小（组均值分别为19.8±1.1和14.8±1.2μm），3级细胞的L和S最大（组均值分别为51.2±7.2和37.2±4.7μm），不同细胞分级的L:S比值无差异。细胞核最长尺寸（NL）值的范围为8.7至14.5μm，0级细胞的组均值为9.8±0.6μm，3级细胞的组均值为12.9±1.2μm。NL与L呈非线性相关。结膜细胞和细胞核尺寸的测量可用于客观区分被指定为不同鳞状化生分级的细胞，细胞核轻度增大是最早可检测到的变化（在0.5级）。
